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ATP5D Antibody Pair

CSB-EAP00635
Cusabio
ApplicationsELISA
Product group Antibodies
ReactivityBovine, Equine, Guinea Pig, Goat, Human, Porcine
TargetATP5F1D
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Overview

  • Supplier
    Cusabio
  • Product Name
    ATP5D Antibody Pair
  • Delivery Days Customer
    9
  • Application Supplier Note
    We recommend using the capture antibody at a concentration of 0.5ug/ml and the detection antibody at a concentration of 0.25ug/ml.Optimal dilutions should be determined experimentally by the researcher.
  • Applications
    ELISA
  • Certification
    Research Use Only
  • Conjugate
    Biotin
  • Gene ID513
  • Target name
    ATP5F1D
  • Target description
    ATP synthase F1 subunit delta
  • Target synonyms
    ATP synthase subunit delta, mitochondrial; ATP synthase, H+ transporting, mitochondrial F1 complex, delta subunit; ATP5D; F-ATPase delta subunit; MC5DN5; mitochondrial ATP synthase complex delta-subunit precusor; mitochondrial ATP synthase, delta subunit
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP30049
  • Protein Name
    ATP synthase subunit delta, mitochondrial
  • Reactivity
    Bovine, Equine, Guinea Pig, Goat, Human, Porcine
  • UNSPSC
    41116163

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Figure 1. Western blot analysis of ATP5D/ATP5F1D using anti-ATP5D/ATP5F1D antibody (A32272-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human Raji whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat kidney tissue lysates, Lane 7: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATP5D/ATP5F1D antigen affinity purified polyclonal antibody (Catalog # A32272-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATP5D/ATP5F1D at approximately 17 kDa. The expected band size for ATP5D/ATP5F1D is at 17 kDa.
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