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ProductsBack/ATRX (C-term) Mouse Monoclonal Antibody [Clone ID: 60.1]
Kidney
Kidney
Kidney

ATRX (C-term) Mouse Monoclonal Antibody [Clone ID: 60.1]

AM06038PU-N
OriGene
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetATRX
Price on request
50 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    ATRX (C-term) Mouse Monoclonal Antibody [Clone ID: 60.1]
  • Delivery Days Customer
    14
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    60.1
  • Gene ID546
  • Target name
    ATRX
  • Target description
    ATRX chromatin remodeler
  • Target synonyms
    JMS, MRX52, RAD54, RAD54L, XH2, XNP, ZNF-HX, transcriptional regulator ATRX, ATP-dependent helicase ATRX, X-linked helicase II, X-linked nuclear protein
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP46100
  • Protein Name
    Transcriptional regulator ATRX
  • Scientific Description
    ATRX (C-term) mouse monoclonal antibody, clone 60.1, Purified
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ATRX using anti-ATRX antibody (A00203-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A54 whole cell lysates, Lane 2: human Hela whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATRX antigen affinity purified polyclonal antibody (Catalog # A00203-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATRX at approximately 282KD. The expected band size for ATRX is at 282KD.
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Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATRX antibody [C3], C-term (GTX101310) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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