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IHC analysis of human tonsil tissue using GTX31252 BAFF Receptor antibody.
IHC analysis of human tonsil tissue using GTX31252 BAFF Receptor antibody.
IHC analysis of human tonsil tissue using GTX31252 BAFF Receptor antibody.

BAFF Receptor antibody

GTX31252
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetTNFRSF13C
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Overview

  • Supplier
    GeneTex
  • Product Name
    BAFF Receptor antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 2-5microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID115650
  • Target name
    TNFRSF13C
  • Target description
    TNF receptor superfamily member 13C
  • Target synonyms
    B cell-activating factor receptor; BAFF receptor; BAFFR; BAFF-R; BLyS receptor 3; BROMIX; CD268; CVID4; prolixin; tumor necrosis factor receptor superfamily member 13C
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96RJ3
  • Protein Name
    Tumor necrosis factor receptor superfamily member 13C
  • Scientific Description
    B cell-activating factor (BAFF) enhances B-cell survival in vitro and is a regulator of the peripheral B-cell population. Overexpression of Baff in mice results in mature B-cell hyperplasia and symptoms of systemic lupus erythematosus (SLE). Also, some SLE patients have increased levels of BAFF in serum. Therefore, it has been proposed that abnormally high levels of BAFF may contribute to the pathogenesis of autoimmune diseases by enhancing the survival of autoreactive B cells. The protein encoded by this gene is a receptor for BAFF and is a type III transmembrane protein containing a single extracellular cysteine-rich domain. It is thought that this receptor is the principal receptor required for BAFF-mediated mature B-cell survival. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of BAFF Receptor using anti-BAFF Receptor antibody (A02865-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: rat spleen tissue lysates, Lane 3: rat thymus tissue lysates, Lane 4: mouse spleen tissue lysates, Lane 5: mouse thymus tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BAFF Receptor antigen affinity purified polyclonal antibody (Catalog # A02865-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BAFF Receptor at approximately 19KD. The expected band size for BAFF Receptor is at 19KD.
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