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ProductsBack/BCA1 antibody [19N1]

BCA1 antibody [19N1]

GTX53296
GeneTex
ApplicationsWestern Blot, Neutralisation/Blocking
Product group Antibodies
ReactivityMouse
TargetCxcl13
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Overview

  • Supplier
    GeneTex
  • Product Name
    BCA1 antibody [19N1]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:250 - 1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, Neutralisation/Blocking
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    19N1
  • Concentration
    500 ug/ml
  • Conjugate
    Unconjugated
  • Gene ID55985
  • Target name
    Cxcl13
  • Target description
    C-X-C motif chemokine ligand 13
  • Target synonyms
    4631412M08Rik, ANGIE2, Angie, BCA-1, BLC, BLR1L, Scyb13, C-X-C motif chemokine 13, B lymphocyte chemoattractant, CXC chemokine BLC, chemokine (C-X-C motif) ligand 13, small inducible cytokine subfamily B (Cys-X-Cys), member 13, small-inducible cytokine B13
  • Host
    Rat
  • Isotype
    IgG2
  • Protein IDO55038
  • Protein Name
    C-X-C motif chemokine 13
  • Reactivity
    Mouse
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CXCL13/BLC using anti-CXCL13/BLC antibody (PB9999). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Recombinant mouse CXCL13/BLC protein 1ng. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CXCL13/BLC antigen affinity purified polyclonal antibody (Catalog # PB9999) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CXCL13/BLC at approximately 13 kDa. The expected band size for CXCL13/BLC is at 12 kDa.
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