More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/BCKDH kinase (BCKDK) Mouse Monoclonal Antibody [Clone ID: OTI11C9]
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY BCKDK (Cat# RC203601, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BCKDK(Cat# TA500685). Positive lysates [LY401780] (100ug) and [LC401780] (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY BCKDK (Cat# RC203601, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BCKDK(Cat# TA500685). Positive lysates [LY401780] (100ug) and [LC401780] (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY BCKDK (Cat# RC203601, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BCKDK(Cat# TA500685). Positive lysates [LY401780] (100ug) and [LC401780] (20ug) can be purchased separately from OriGene.

BCKDH kinase (BCKDK) Mouse Monoclonal Antibody [Clone ID: OTI11C9]

CF500685
OriGene
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetBCKDK
Price on request
100 ug
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    OriGene
  • Product Name
    BCKDH kinase (BCKDK) Mouse Monoclonal Antibody [Clone ID: OTI11C9]
  • Delivery Days Customer
    14
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI11C9
  • Gene ID10295
  • Target name
    BCKDK
  • Target description
    branched chain keto acid dehydrogenase kinase
  • Target synonyms
    BCKDKD, BDK, branched-chain alpha-ketoacid dehydrogenase kinase, BCKD-kinase, BCKDH kinase, BCKDHKIN, [3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrial, branched chain alpha-ketoacid dehydrogenase kinase, branched chain ketoacid dehydrogenase kinase
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDO14874
  • Protein Name
    Branched-chain alpha-ketoacid dehydrogenase kinase
  • Scientific Description
    Carrier-free (BSA/glycerol-free) BCKDK mouse monoclonal antibody, clone OTI11C9 (formerly 11C9)
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

Download MSDS

FAQ

Go to our frequently asked questions

Related products

Western Blot analysis of 3T3 cells using BCKDK Polyclonal Antibody
Product group Antibodies
Cusabio
BCKDK AntibodyCSB-PA000981
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetBCKDK
  • SizePrice
Product group Antibodies
Assay Genie
BCKDK AntibodyCAB8184
ReactivityHuman
TargetBCKDK
  • SizePrice
Figure 1. Western blot analysis of BCKDK using anti-BCKDK antibody (A07798-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: rat kidney tissue lysates, Lane 3: mouse kidney tissue lysates, Lane 4: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BCKDK antigen affinity purified polyclonal antibody (Catalog # A07798-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BCKDK at approximately 46 kDa. The expected band size for BCKDK is at 46 kDa.
Product group Antibodies
Boster Bio
Anti-BCKDK Antibody Picoband(r)A07798-2-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetBCKDK
  • SizePrice
Product group Antibodies
RayBiotech
Anti-BCKDK Antibody144-08184
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetBCKDK
  • SizePrice
Anti-BCKDK (E41) Antibody
Product group Antibodies
Antibodies.com
Anti-BCKDK (E41) AntibodyA25858
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
  • SizePrice
Product group Antibodies
MedChem Express
BCKDK Antibody (YA2737, Rabbit)HY-P82992
TargetBCKDK
  • SizePrice
Immunohistochemical staining of human Fallopian tube shows strong granular cytoplasmic positivity in glandular cells.
Product group Antibodies
Atlas Antibodies
Anti-BCKDK AntibodyHPA017995
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetBCKDK
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
BCKDK AntibodyLS-C409720
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetBCKDK
  • SizePrice
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with BCKDK antibody [N2C3] (GTX104477) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Product group Antibodies
GeneTex
BCKDK antibody [N2C3]GTX104477
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Rat
TargetBCKDK
  • SizePrice
Product group Antibodies
Biosynth
BCKDK antibody70R-50786
  • SizePrice