Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with BCKDK antibody [HL2710] (GTX639471) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![Wild-type (WT) and BCKDK knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with BCKDK antibody [HL2710] (GTX639471) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX639471/GTX639471_T-45250_20231222_WB_KO_watermark_23122619_364.webp "Wild-type (WT) and BCKDK knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with BCKDK antibody [HL2710] (GTX639471) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![BCKDK antibody [HL2710] detects BCKDK protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. BCKDK stained by BCKDK antibody [HL2710] (GTX639471) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX639471/GTX639471_T-45250_20240112_IHC-P_M_24021917_450.webp "BCKDK antibody [HL2710] detects BCKDK protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. BCKDK stained by BCKDK antibody [HL2710] (GTX639471) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with BCKDK antibody [HL2710] (GTX639471) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](https://www.genetex.com/upload/website/prouct_img/normal/GTX639471/GTX639471_45320_20240223_WB_TPM_watermark_24022619_603.webp "Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with BCKDK antibody [HL2710] (GTX639471) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with BCKDK antibody [HL2710] (GTX639471) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
BCKDK antibody [HL2710]
GTX639471
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetBCKDK
Overview
- SupplierGeneTex
- Product NameBCKDK antibody [HL2710]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL2710
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID10295
- Target nameBCKDK
- Target descriptionbranched chain keto acid dehydrogenase kinase
- Target synonymsBCKDKD, BDK, branched-chain alpha-ketoacid dehydrogenase kinase, BCKD-kinase, BCKDH kinase, BCKDHKIN, [3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrial, branched chain alpha-ketoacid dehydrogenase kinase, branched chain ketoacid dehydrogenase kinase
- HostRabbit
- IsotypeIgG
- Protein IDO14874
- Protein NameBranched-chain alpha-ketoacid dehydrogenase kinase
- Scientific DescriptionThe branched-chain alpha-ketoacid dehydrogenase complex (BCKD) is an important regulator of the valine, leucine, and isoleucine catabolic pathways. The protein encoded by this gene is found in the mitochondrion, where it phosphorylates and inactivates BCKD. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2012]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
Datasheet
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