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IHC-P analysis of human heart tissue using GTX89376 beta 1 Adrenergic Receptor antibody, Internal. Antigen retrieval : Heat induced antigen retrieval with citrate buffer pH 6
IHC-P analysis of human heart tissue using GTX89376 beta 1 Adrenergic Receptor antibody, Internal. Antigen retrieval : Heat induced antigen retrieval with citrate buffer pH 6
IHC-P analysis of human heart tissue using GTX89376 beta 1 Adrenergic Receptor antibody, Internal. Antigen retrieval : Heat induced antigen retrieval with citrate buffer pH 6

beta 1 Adrenergic Receptor antibody, Internal

GTX89376
GeneTex
ApplicationsImmunoFluorescence, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetADRB1
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Overview

  • Supplier
    GeneTex
  • Product Name
    beta 1 Adrenergic Receptor antibody, Internal
  • Delivery Days Customer
    9
  • Applications
    ImmunoFluorescence, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.50 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID153
  • Target name
    ADRB1
  • Target description
    adrenoceptor beta 1
  • Target synonyms
    ADRB1R, B1AR, BETA1AR, FNSS2, RHR, beta-1 adrenergic receptor, adrenergic, beta-1-, receptor, beta-1 adrenoceptor, beta-1 adrenoreceptor
  • Host
    Goat
  • Isotype
    IgG
  • Protein IDP08588
  • Protein Name
    Beta-1 adrenergic receptor
  • Scientific Description
    The adrenergic receptors (subtypes alpha 1, alpha 2, beta 1, and beta 2) are a prototypic family of guanine nucleotide binding regulatory protein-coupled receptors that mediate the physiological effects of the hormone epinephrine and the neurotransmitter norepinephrine. Specific polymorphisms in this gene have been shown to affect the resting heart rate and can be involved in heart failure. [provided by RefSeq, Jul 2008]
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ADRB1 using anti-ADRB1 antibody (A00675-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: rat NRK whole cell lysates, Lane 3: mouse heart tissue lysates, Lane 4: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADRB1 antigen affinity purified polyclonal antibody (Catalog # A00675-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ADRB1 at approximately 51 kDa. The expected band size for ADRB1 is at 51 kDa.
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