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WB analysis of HL-60 cell lysate using GTX32471 Bid antibody. Loading : 25microg per lane
WB analysis of HL-60 cell lysate using GTX32471 Bid antibody. Loading : 25microg per lane
WB analysis of HL-60 cell lysate using GTX32471 Bid antibody. Loading : 25microg per lane

Bid antibody

GTX32471
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
TargetBID
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Overview

  • Supplier
    GeneTex
  • Product Name
    Bid antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:2000. ICC/IF: 1:50 - 1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID637
  • Target name
    BID
  • Target description
    BH3 interacting domain death agonist
  • Target synonyms
    apoptic death agonist; BH3-interacting domain death agonist; desmocollin type 4; FP497; Human BID coding sequence; p22 BID
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP55957
  • Protein Name
    BH3-interacting domain death agonist
  • Scientific Description
    This gene encodes a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. The encoded protein is a member of the BCL-2 family of cell death regulators. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 2. IHC analysis of BID using anti-BID antibody (PB9027). BID was detected in paraffin-embedded section of mouse spleen tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1microg/ml rabbit anti-BID Antibody (PB9027) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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