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Biotin Anti-Human CD18 Antibody

RD20749F
Reddot Biotech
ApplicationsFlow Cytometry
Product group Antibodies
ReactivityHuman
TargetITGB2
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Overview

  • Supplier
    Reddot Biotech
  • Product Name
    Biotin Anti-Human CD18 Antibody
  • Delivery Days Customer
    5
  • Applications
    Flow Cytometry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    TS1/18.1.2.11
  • Concentration
    0.5 mg/ml
  • Conjugate
    Biotin
  • Gene ID3689
  • Target name
    ITGB2
  • Target description
    integrin subunit beta 2
  • Target synonyms
    CD18, LAD, LCAMB, LFA-1, MAC-1, MF17, MFI7, integrin beta-2, cell surface adhesion glycoprotein (LFA-1/CR3/P150,959 beta subunit precursor), complement component 3 receptor 3 and 4 subunit, complement receptor C3 beta-subunit, integrin beta chain, beta 2, integrin, beta 2 (complement component 3 receptor 3 and 4 subunit), leukocyte cell adhesion molecule CD18, leukocyte-associated antigens CD18/11A, CD18/11B, CD18/11C
  • Host
    Mouse
  • Isotype
    IgG1
  • Scientific Description
    CD18 is a 90-95 kD type I transmembrane protein also known as integrin beta2 subunit, LFA-1 beta subunit, and beta2 integrin. CD18 non-covalently associates with CD11a, CD11b or CD11c. CD18 is expressed on all leukocytes. CD18 and associated Oe+/- chains function in adhesion and signaling in hematopoietic cells.
  • Reactivity
    Human
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

FAQ

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Figure 1. Western blot analysis of CD18/ITGB2 using anti-CD18/ITGB2 antibody (PB9141). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD18/ITGB2 antigen affinity purified polyclonal antibody (Catalog # PB9141) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD18/ITGB2 at approximately 90-100 kDa. The expected band size for CD18/ITGB2 is at 85 kDa.
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