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Mouse anti Human BMI1

MUB2004P
Nordic-MUbio
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
Product group Antibodies
TargetBMI1
0.1 mg
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Overview

  • Supplier
    Nordic-MUbio
  • Product Name
    Mouse anti Human BMI1
  • Delivery Days Customer
    7
  • Application Supplier Note
    229F6 is useful for immunoprecipitation, western blot and immunohistochemistry on frozen sections. Optimal antibody dilution should be determined by titration.
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
  • Applications Supplier
    Immunohistochemistry (frozen);Immunoprecipitation;Western Blotting
  • Category Supplier
    Primary antibodies
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    229F6
  • Gene ID648
  • Target name
    BMI1
  • Target description
    BMI1 proto-oncogene, polycomb ring finger
  • Target synonyms
    B lymphoma Mo-MLV insertion region 1 homolog; BMI1 polycomb ring finger oncogene; BMI1 polycomb ring finger proto-oncogene; FLVI2/BMI1; flvi-2/bmi-1; murine leukemia viral (bmi-1) oncogene homolog; PCGF4; polycomb complex protein BMI-1; polycomb group protein Bmi1; polycomb group RING finger protein 4; ring finger protein 51; RNF51
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP35226
  • Protein Name
    Polycomb complex protein BMI-1
  • Source
    229F6 is a Mouse monoclonal IgG1 antibody derived by fusion of SP2/0 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with recombinant Bmi1 protein corresponding to residues 1-202 of Mouse Bmi-1 acterial fusion protein of membrane ...
  • Reactivity Supplier
    Human;Mouse;Rabbit;Rat
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of BMI1 using anti-BMI1 antibody (PB9133). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human U2O whole cell lysates Lane 2: human PC-3 whole cell lysates Lane 3: human HEK293 whole cell lysates Lane 4: human Caco-2 whole cell lysates After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BMI1 antigen affinity purified polyclonal antibody (Catalog # PB9133) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BMI1 at approximately 43-45KD. The expected band size for BMI1 is at 39KD.
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