More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/BUB1 antibody
WB analysis of various sample lysates using GTX30097 BUB1 antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX30097 BUB1 antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX30097 BUB1 antibody. Dilution : 1:1000 Loading : 25microg per lane

BUB1 antibody

GTX30097
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetBUB1
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    GeneTex
  • Product Name
    BUB1 antibody
  • Delivery Days Customer
    7
  • Application Supplier Note
    WB: 1:500 - 1:2000. IHC-P: 1:50 - 1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID699
  • Target name
    BUB1
  • Target description
    BUB1 mitotic checkpoint serine/threonine kinase
  • Target synonyms
    BUB1 budding uninhibited by benzimidazoles 1 homolog; BUB1A; BUB1L; budding uninhibited by benzimidazoles 1 homolog; hBUB1; mitotic checkpoint serine/threonine-protein kinase BUB1; mitotic spindle checkpoint kinase; putative serine/threonine-protein kinase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO43683
  • Protein Name
    Mitotic checkpoint serine/threonine-protein kinase BUB1
  • Scientific Description
    This gene encodes a serine/threonine-protein kinase that play a central role in mitosis. The encoded protein functions in part by phosphorylating members of the mitotic checkpoint complex and activating the spindle checkpoint. This protein also plays a role in inhibiting the activation of the anaphase promoting complex/cyclosome. This protein may also function in the DNA damage response. Mutations in this gene have been associated with aneuploidy and several forms of cancer. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

Related products

Product group Antibodies
Biomatik
Bub1 Recombinant AntibodyCAC12031
ApplicationsWestern Blot, ELISA
TargetBUB1
  • SizePrice
Product group Antibodies
Assay Genie
CIRBP Monoclonal AntibodyCAB19290
TargetBUB1
  • SizePrice
Product group Antibodies
Antibodies.com
Anti-BUB1 AntibodyA101535
ApplicationsWestern Blot, ELISA
  • SizePrice
Product group Antibodies
GeneTex
BUB1 antibody [14H5]GTX80316
ApplicationsImmunoFluorescence, ImmunoPrecipitation, ImmunoCytoChemistry
TargetBUB1
  • SizePrice
WB analysis of A-20 cell lysate using GTX31758 BUB1 antibody. Working concentration : (A) 2 and (B) 4 microg/ml
Product group Antibodies
GeneTex
BUB1 antibodyGTX31758
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
TargetBUB1
  • SizePrice
Product group Antibodies
References
GeneTex
BUB1 antibodyGTX107497
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
TargetBUB1
  • SizePrice
Product group Antibodies
MedChem Express
BUB1 Antibody (YA1567)HY-P81822
TargetBUB1
  • SizePrice
Immunohistochemistry of paraffin-embedded human gastric cancer using CSB-PA002882LA01HU at dilution of 1:100
Product group Antibodies
Cusabio
BUB1 AntibodyCSB-PA002882LA01HU
ApplicationsELISA, ImmunoHistoChemistry
TargetBUB1
  • SizePrice
Figure 1. Western blot analysis of BUB1 using anti-BUB1 antibody (A00776-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BUB1 antigen affinity purified polyclonal antibody (Catalog # A00776-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BUB1 at approximately 130 kDa. The expected band size for BUB1 is at 122 kDa.
Product group Antibodies
Boster Bio
Anti-BUB1 Antibody Picoband(r)A00776-3-CARRIER-FREE
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
TargetBUB1
  • SizePrice
Product group Antibodies
Bethyl Laboratories
Rabbit anti-Bub1 Antibody, Affinity PurifiedA300-373A
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
TargetBUB1
  • SizePrice