WB (peptide competition) analysis of fibroblasts transfected with oncogenic DSH3-Abl using GTX24479 c-Abl (phospho Tyr245) antibody with the non-phosphopeptide corresponding to the phosphopeptide immunogen (Lane 2), a generic phosphothreonine-containing peptide (Lane 3), or the phosphopeptide immunogen (Lane 4) control. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.
WB (peptide competition) analysis of fibroblasts transfected with oncogenic DSH3-Abl using GTX24479 c-Abl (phospho Tyr245) antibody with the non-phosphopeptide corresponding to the phosphopeptide immunogen (Lane 2), a generic phosphothreonine-containing peptide (Lane 3), or the phosphopeptide immunogen (Lane 4) control. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.
c-Abl (phospho Tyr245) antibody
GTX24479
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetABL1
Overview
- SupplierGeneTex
- Product Namec-Abl (phospho Tyr245) antibody - Orthogonal Validated
- Delivery Days Customer9
- Antibody SpecificityThis antibody is raised against human c-Abl (isoform b) phosphorylated at Tyr245. Based on sequence homology, it is predicted to react with c-Abl (isoform a) and ABL2 when phosphorylated at the corresponding residues - c-Abl (isoform a) Tyr226 or ABL2 Tyr
- Application Supplier NoteWB: 0.1-1 microg/ml. ICC/IF: 1:250. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- ConjugateUnconjugated
- Gene ID25
- Target nameABL1
- Target descriptionABL proto-oncogene 1, non-receptor tyrosine kinase
- Target synonymsAbelson tyrosine-protein kinase 1; ABL; ABL protooncogene 1 nonreceptor tyrosine kinase; bcr/abl; bcr/c-abl oncogene protein; BCR-ABL; c-ABL; c-abl oncogene 1, receptor tyrosine kinase; c-ABL1; CHDSKM; JTK7; p150; proto-oncogene c-Abl; proto-oncogene tyrosine-protein kinase ABL1; truncated ABL protooncogene 1 nonreceptor tyrosine kinase; tyrosine-protein kinase ABL1; v-abl; v-abl Abelson murine leukemia viral oncogene homolog 1
- HostRabbit
- IsotypeIgG
- Protein IDP00519
- Protein NameTyrosine-protein kinase ABL1
- Scientific DescriptionThis gene is a protooncogene that encodes a protein tyrosine kinase involved in a variety of cellular processes, including cell division, adhesion, differentiation, and response to stress. The activity of the protein is negatively regulated by its SH3 domain, whereby deletion of the region encoding this domain results in an oncogene. The ubiquitously expressed protein has DNA-binding activity that is regulated by CDC2-mediated phosphorylation, suggesting a cell cycle function. This gene has been found fused to a variety of translocation partner genes in various leukemias, most notably the t(9;22) translocation that results in a fusion with the 5 end of the breakpoint cluster region gene (BCR; MIM:151410). Alternative splicing of this gene results in two transcript variants, which contain alternative first exons that are spliced to the remaining common exons. [provided by RefSeq, Aug 2014]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203