Wild-type (WT) and c-Met knockout (KO) HeLa cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with c-Met antibody [C3], C-term (GTX100637) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![Immunoprecipitation of c-Met protein from HeLa whole cell extracts using 5 μg of c-Met antibody [C3], C-term (GTX100637). Western blot analysis was performed using c-Met antibody [C3], C-term (GTX100637) diluted at 1:500. EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.](https://www.genetex.com/upload/website/prouct_img/normal/GTX100637/GTX100637_39988_IP_w_23060100_769.webp "Immunoprecipitation of c-Met protein from HeLa whole cell extracts using 5 μg of c-Met antibody [C3], C-term (GTX100637). Western blot analysis was performed using c-Met antibody [C3], C-term (GTX100637) diluted at 1:500. EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.")
![c-Met antibody [c3], c-term detects c-Met protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: c-Met protein stained by c-Met antibody [c3], c-term (GTX100637) diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500. Blue: Hoechst 33342 staining.](https://www.genetex.com/upload/website/prouct_img/normal/GTX100637/GTX100637_39988_20150410_IFA_w_23060100_796.webp "c-Met antibody [c3], c-term detects c-Met protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: c-Met protein stained by c-Met antibody [c3], c-term (GTX100637) diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500. Blue: Hoechst 33342 staining.")
![Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with c-Met antibody [C3], C-term (GTX100637) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX100637/GTX100637_39988_20160901_WB_shRNA_watermark_w_23060100_148.webp "Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with c-Met antibody [C3], C-term (GTX100637) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with c-Met antibody [C3], C-term (GTX100637) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](https://www.genetex.com/upload/website/prouct_img/normal/GTX100637/GTX100637_43901_20200424_WB_w_23060100_713.webp "Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with c-Met antibody [C3], C-term (GTX100637) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
Wild-type (WT) and c-Met knockout (KO) HeLa cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with c-Met antibody [C3], C-term (GTX100637) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
c-Met antibody [C3], C-term
GTX100637
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetMET
Overview
- SupplierGeneTex
- Product Namec-Met antibody [C3], C-term
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1.95 mg/ml
- ConjugateUnconjugated
- Gene ID4233
- Target nameMET
- Target descriptionMET proto-oncogene, receptor tyrosine kinase
- Target synonymsAUTS9, DA11, DFNB97, HGFR, RCCP2, c-Met, hepatocyte growth factor receptor, HGF receptor, HGF/SF receptor, SF receptor, proto-oncogene c-Met, scatter factor receptor, tyrosine-protein kinase Met
- HostRabbit
- IsotypeIgG
- Protein IDP08581
- Protein NameHepatocyte growth factor receptor
- Scientific DescriptionThe proto-oncogene MET product is the hepatocyte growth factor receptor and encodes tyrosine-kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Various mutations in the MET gene are associated with papillary renal carcinoma. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
Datasheet
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