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Formalin-fixed and paraffin-embedded mouse intestine labeled with Anti-C-Met/Met/HGFR Polyclonal Antibody, Unconjugated(bs-0668R) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C.
Formalin-fixed and paraffin-embedded mouse intestine labeled with Anti-C-Met/Met/HGFR Polyclonal Antibody, Unconjugated(bs-0668R) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C.
Formalin-fixed and paraffin-embedded mouse intestine labeled with Anti-C-Met/Met/HGFR Polyclonal Antibody, Unconjugated(bs-0668R) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C.

MET Polyclonal Antibody

BS-0668R
Bioss
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetMET
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Overview

  • Supplier
    Bioss
  • Product Name
    MET Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID4233
  • Target name
    MET
  • Target description
    MET proto-oncogene, receptor tyrosine kinase
  • Target synonyms
    AUTS9, DA11, DFNB97, HGFR, RCCP2, c-Met, hepatocyte growth factor receptor, HGF receptor, HGF/SF receptor, SF receptor, proto-oncogene c-Met, scatter factor receptor, tyrosine-protein kinase Met
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP08581
  • Protein Name
    Hepatocyte growth factor receptor
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Wang X, Tang Y, Shen R, et al. Hepatocyte growth factor (HGF) optimizes oral traumatic ulcer healing of mice by reducing inflammation. Cytokine. 2017,99:275-280. doi: 10.1016/j.cyto.2017.08.006
    Read this paper
  • Qi Y, Li M, Xu L, et al. Therapeutic role of human hepatocyte growth factor (HGF) in treating hair loss. PeerJ. 2016,4:e2624. doi: 10.7717/peerj.2624
    Read this paper
  • Li H, Zhang H, Zhao S, et al. Overexpression of MACC1 and the association with hepatocyte growth factor/c-Met in epithelial ovarian cancer. Oncol Lett. 2015,9(5):1989-1996.
    Read this paper
  • Guo T, Yang J, Yao J, et al. Expression of MACC1 and c-Met in human gastric cancer and its clinical significance. Cancer Cell Int. 2013,13(1):121. doi: 10.1186/1475-2867-13-121
    Read this paper

Datasheet

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Figure 1. Western blot analysis of Met (c-Met) using anti-Met (c-Met) antibody (PB9186). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Met (c-Met) antigen affinity purified polyclonal antibody (Catalog # PB9186) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Met (c-Met) at approximately 154 kDa. The expected band size for Met (c-Met) is at 156 kDa.
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