c-Met antibody detects c-Met protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with c-Met antibody (GTX631993) diluted by 1:1000.
![Immunoprecipitation of c-Met protein from HeLa whole cell extracts using 5 μg of c-Met antibody [GT1586] (GTX631993). Western blot analysis was performed using c-Met antibody [GT1586] (GTX631993). EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.](https://www.genetex.com/upload/website/prouct_img/normal/GTX631993/GTX631993_41904_20150710_IP_w_23061202_300.webp "Immunoprecipitation of c-Met protein from HeLa whole cell extracts using 5 μg of c-Met antibody [GT1586] (GTX631993). Western blot analysis was performed using c-Met antibody [GT1586] (GTX631993). EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.")
![Wild-type (WT) and c-Met knockout (KO) HeLa cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with c-Met antibody [GT1586] (GTX631993) diluted at 1:2000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](https://www.genetex.com/upload/website/prouct_img/normal/GTX631993/GTX631993_41904_20170601_WB_KO_watermark_w_23061202_426.webp "Wild-type (WT) and c-Met knockout (KO) HeLa cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with c-Met antibody [GT1586] (GTX631993) diluted at 1:2000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with c-Met antibody [GT1586] (GTX631993) diluted at 1:500.](https://www.genetex.com/upload/website/prouct_img/normal/GTX631993/GTX631993_41904_20160901_WB_shRNA_watermark_w_23061202_294.webp "Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with c-Met antibody [GT1586] (GTX631993) diluted at 1:500.")
c-Met antibody detects c-Met protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with c-Met antibody (GTX631993) diluted by 1:1000.
c-Met antibody [GT1586]
GTX631993
ApplicationsImmunoPrecipitation, Western Blot
Product group Antibodies
ReactivityHuman
TargetMET
Overview
- SupplierGeneTex
- Product Namec-Met antibody [GT1586]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoPrecipitation, Western Blot
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDGT1586
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID4233
- Target nameMET
- Target descriptionMET proto-oncogene, receptor tyrosine kinase
- Target synonymsAUTS9, DA11, DFNB97, HGFR, RCCP2, c-Met, hepatocyte growth factor receptor, HGF receptor, HGF/SF receptor, SF receptor, proto-oncogene c-Met, scatter factor receptor, tyrosine-protein kinase Met
- HostMouse
- IsotypeIgG1
- Protein IDP08581
- Protein NameHepatocyte growth factor receptor
- Scientific DescriptionThe proto-oncogene MET product is the hepatocyte growth factor receptor and encodes tyrosine-kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Various mutations in the MET gene are associated with papillary renal carcinoma. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
Datasheet
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![Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with c-Met antibody [GT556] (GTX631992) diluted at 1:500.](https://www.genetex.com/upload/website/prouct_img/normal/GTX631992/GTX631992_41904_20160901_WB_shRNA_watermark_w_23061202_797.webp)
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