Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with c-Met antibody [GT556] (GTX631992) diluted at 1:500.
![Immunoprecipitation of c-Met protein from HeLa whole cell extracts using 5 μg of MET antibody [GT556] (GTX631992). Western blot analysis was performed using c-Met antibody [GT556] (GTX631992). EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.](https://www.genetex.com/upload/website/prouct_img/normal/GTX631992/GTX631992_41904_20150710_IP_w_23061202_186.webp "Immunoprecipitation of c-Met protein from HeLa whole cell extracts using 5 μg of MET antibody [GT556] (GTX631992). Western blot analysis was performed using c-Met antibody [GT556] (GTX631992). EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.")
![Wild-type (WT) and c-Met knockout (KO) HeLa cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with c-Met antibody [GT556] (GTX631992) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](https://www.genetex.com/upload/website/prouct_img/normal/GTX631992/GTX631992_41904_20170601_WB_KO_watermark_w_23061202_892.webp "Wild-type (WT) and c-Met knockout (KO) HeLa cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with c-Met antibody [GT556] (GTX631992) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with c-Met antibody [GT556] (GTX631992) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX631992/GTX631992_44699_20220527_WB_25061003_958.webp "Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with c-Met antibody [GT556] (GTX631992) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.")
Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with c-Met antibody [GT556] (GTX631992) diluted at 1:500.
c-Met antibody [GT556]
GTX631992
ApplicationsFlow Cytometry, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetMET
Overview
- SupplierGeneTex
- Product Namec-Met antibody [GT556]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDGT556
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID4233
- Target nameMET
- Target descriptionMET proto-oncogene, receptor tyrosine kinase
- Target synonymsAUTS9, DA11, DFNB97, HGFR, RCCP2, c-Met, hepatocyte growth factor receptor, HGF receptor, HGF/SF receptor, SF receptor, proto-oncogene c-Met, scatter factor receptor, tyrosine-protein kinase Met
- HostMouse
- IsotypeIgG2a
- Protein IDP08581
- Protein NameHepatocyte growth factor receptor
- Scientific DescriptionThe proto-oncogene MET product is the hepatocyte growth factor receptor and encodes tyrosine-kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Various mutations in the MET gene are associated with papillary renal carcinoma. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Hung YH, Lai MD, Hung WC, et al. Semaphorin 4C promotes motility and immunosuppressive activity of cancer cells via CRMP3 and PD-L1. Am J Cancer Res. 2022,12(2):713-728.Read this paper
Datasheet
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