Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with c-Met (phospho Tyr1234) antibody [HL2907] (GTX640244) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
![Specificity test was performed by coating the plate with MET peptide, MET (phospho Tyr1234) peptide, MET (phospho Tyr1235) peptide, and MET (phospho Tyr1234/Tyr1235) peptide (15.63 ng/mL). Coated peptides were probed with c-Met (phospho Tyr1234) antibody [HL2907] (GTX640244) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX640244/GTX640244_45439_20241122_ELISA_Indirect_Multiple_Bar_Specificity_24120522_863.webp "Specificity test was performed by coating the plate with MET peptide, MET (phospho Tyr1234) peptide, MET (phospho Tyr1235) peptide, and MET (phospho Tyr1234/Tyr1235) peptide (15.63 ng/mL). Coated peptides were probed with c-Met (phospho Tyr1234) antibody [HL2907] (GTX640244) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.")
Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with c-Met (phospho Tyr1234) antibody [HL2907] (GTX640244) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
c-Met (phospho Tyr1234) antibody [HL2907]
GTX640244
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetMET
Overview
- SupplierGeneTex
- Product Namec-Met (phospho Tyr1234) antibody [HL2907]
- Delivery Days Customer7
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL2907
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID4233
- Target nameMET
- Target descriptionMET proto-oncogene, receptor tyrosine kinase
- Target synonymsAUTS9, DA11, DFNB97, HGFR, RCCP2, c-Met, hepatocyte growth factor receptor, HGF receptor, HGF/SF receptor, SF receptor, proto-oncogene c-Met, scatter factor receptor, tyrosine-protein kinase Met
- HostRabbit
- IsotypeIgG
- Protein IDP08581
- Protein NameHepatocyte growth factor receptor
- Scientific DescriptionThis gene encodes a member of the receptor tyrosine kinase family of proteins and the product of the proto-oncogene MET. The encoded preproprotein is proteolytically processed to generate alpha and beta subunits that are linked via disulfide bonds to form the mature receptor. Further processing of the beta subunit results in the formation of the M10 peptide, which has been shown to reduce lung fibrosis. Binding of its ligand, hepatocyte growth factor, induces dimerization and activation of the receptor, which plays a role in cellular survival, embryogenesis, and cellular migration and invasion. Mutations in this gene are associated with papillary renal cell carcinoma, hepatocellular carcinoma, and various head and neck cancers. Amplification and overexpression of this gene are also associated with multiple human cancers. [provided by RefSeq, May 2016]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
Datasheet
Related products
Product group Antibodies
Anti-MET [7A2]AB03829-23.0
ApplicationsELISA, Neutralisation/Blocking, Other Application
ReactivityHuman
TargetMET
- SizePrice
Product group Antibodies
c-Met (Phospho-Tyr1003) AntibodyABX012477
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
- SizePrice
Product group Antibodies
C-Met (MET) Polyclonal AntibodyCAU24571
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityMouse
TargetMET
- SizePrice
Product group Antibodies
References
MET Polyclonal AntibodyBS-0668R
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Rat
TargetMET
- SizePrice
Product group Antibodies
Anti-Met AntibodyA101612
ApplicationsWestern Blot, ELISA
ReactivityHuman
- SizePrice
Product group Antibodies
c-Met (phospho Tyr1003) antibodyGTX25656
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityCanine, Hamster, Human, Mouse
TargetMET
- SizePrice
Product group Antibodies
MET AntibodyCSB-PA003231
ApplicationsWestern Blot, ELISA
ReactivityHuman
TargetMET
- SizePrice