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Western Blot analysis of HepG2 cells using Met Polyclonal Antibody
Western Blot analysis of HepG2 cells using Met Polyclonal Antibody
Western Blot analysis of HepG2 cells using Met Polyclonal Antibody

MET Antibody

CSB-PA003231
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetMET
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Overview

  • Supplier
    Cusabio
  • Product Name
    MET Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4233
  • Target name
    MET
  • Target description
    MET proto-oncogene, receptor tyrosine kinase
  • Target synonyms
    AUTS9; c-Met; DFNB97; hepatocyte growth factor receptor; HGF receptor; HGF/SF receptor; HGFR; proto-oncogene c-Met; RCCP2; scatter factor receptor; SF receptor; tyrosine-protein kinase Met
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP08581
  • Protein Name
    Hepatocyte growth factor receptor
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Met (c-Met) using anti-Met (c-Met) antibody (PB9186). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Met (c-Met) antigen affinity purified polyclonal antibody (Catalog # PB9186) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Met (c-Met) at approximately 154 kDa. The expected band size for Met (c-Met) is at 156 kDa.
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