![Indirect ELISA analysis performed by coating plate with recombinant mature C-Peptide protein (19.05-0.3 nM). Coated protein was probed with C-Peptide antibody [HL1158] (GTX636462) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody. Indirect ELISA analysis performed by coating plate with recombinant mature C-Peptide protein (19.05-0.3 nM). Coated protein was probed with C-Peptide antibody [HL1158] (GTX636462) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX636462/GTX636462_44466_20211105_ELISA_Indirect_w_23061202_827.webp)
Indirect ELISA analysis performed by coating plate with recombinant mature C-Peptide protein (19.05-0.3 nM). Coated protein was probed with C-Peptide antibody [HL1158] (GTX636462) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.
C-Peptide antibody [HL1158]
GTX636462
ApplicationsELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetINS
Overview
- SupplierGeneTex
- Product NameC-Peptide antibody [HL1158]
- Delivery Days Customer9
- Application Supplier NoteIHC-P: 1:100-1:10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL1158
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID3630
- Target nameINS
- Target descriptioninsulin
- Target synonymsIDDM, IDDM1, IDDM2, ILPR, IRDN, MODY10, PNDM4, insulin, preproinsulin, proinsulin
- HostRabbit
- IsotypeIgG
- Protein IDP01308
- Protein NameInsulin
- Scientific DescriptionAfter removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. A multitude of mutant alleles with phenotypic effects have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5 region and with the IGF2 gene at the 3 region. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2010]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
![C-Peptide antibody [HL1158] detects C-Peptide protein by immunohistochemical analysis. Sample: Paraffin-embedded rat pancreas. C-Peptide stained by C-Peptide antibody [HL1158] (GTX636462) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min C-Peptide antibody [HL1158] detects C-Peptide protein by immunohistochemical analysis. Sample: Paraffin-embedded rat pancreas. C-Peptide stained by C-Peptide antibody [HL1158] (GTX636462) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX636462/GTX636462_45635_20250214_IHC-P_R_25031220_666.webp)
![C-Peptide antibody [HL1158] detects C-Peptide protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. C-Peptide stained by C-Peptide antibody [HL1158] (GTX636462) diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min C-Peptide antibody [HL1158] detects C-Peptide protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. C-Peptide stained by C-Peptide antibody [HL1158] (GTX636462) diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX636462/GTX636462_45635_20251114_IHC-P_M_Multiple_25112100_459.webp)
![C-Peptide antibody [HL1158] detects C-Peptide protein by immunohistochemical analysis. Sample: Paraffin-embedded human tissues. C-Peptide stained by C-Peptide antibody [HL1158] (GTX636462) diluted at 1:5000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min C-Peptide antibody [HL1158] detects C-Peptide protein by immunohistochemical analysis. Sample: Paraffin-embedded human tissues. C-Peptide stained by C-Peptide antibody [HL1158] (GTX636462) diluted at 1:5000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX636462/GTX636462_45635_20251114_IHC-P_Multiple_RPKM_25112100_464.webp)




![Mouse tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Insulin antibody [GT1229] (GTX02826) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX02826/GTX02826_4000000209_20210122_WB_M_pancreas_w_23053123_391.webp)

![An ELISA plate is coated with 50 μL of a Proinsulin recombinant protein at concentration ranged from 0.2 μg/mL to 1.6 μg/mL. The coated protein is detected with anti-C-peptide antibody [GT1489] (GTX631942) at 500 ng/mL.](https://www.genetex.com/upload/website/prouct_img/normal/GTX631942/GTX631942_41848_20150206_ELISA_w_23061202_525.webp)
![An ELISA plate is coated with 50 μL of a Proinsulin recombinant protein at concentration ranged from 0.2 μg/mL to 1.6 μg/mL. The coated protein is detected with anti-C-peptide antibody [GT1519] (GTX631943) at 500 ng/mL.](https://www.genetex.com/upload/website/prouct_img/normal/GTX631943/GTX631943_41848_20150206_ELISA_w_23061202_907.webp)