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ProductsBack/Calnexin antibody [GT1563]
Calnexin antibody [GT1563] detects Calnexin protein at cytoplasm by immunofluorescent analysis. Sample: Neuro2A cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Calnexin stained by Calnexin antibody [GT1563] (GTX629976) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).
Calnexin antibody [GT1563] detects Calnexin protein at cytoplasm by immunofluorescent analysis. Sample: Neuro2A cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Calnexin stained by Calnexin antibody [GT1563] (GTX629976) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).
Calnexin antibody [GT1563] detects Calnexin protein at cytoplasm by immunofluorescent analysis. Sample: Neuro2A cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Calnexin stained by Calnexin antibody [GT1563] (GTX629976) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).

Calnexin antibody [GT1563]

GTX629976
GeneTex
ApplicationsDot Blot, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCANX
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Overview

  • Supplier
    GeneTex
  • Product Name
    Calnexin antibody [GT1563]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:10000. ICC/IF: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Dot Blot, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    GT1563
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID821
  • Target name
    CANX
  • Target description
    calnexin
  • Target synonyms
    CNX, IP90, P90, calnexin, epididymis secretory sperm binding protein, major histocompatibility complex class I antigen-binding protein p88
  • Host
    Mouse
  • Isotype
    IgG2a
  • Protein IDP27824
  • Protein Name
    Calnexin
  • Scientific Description
    This gene encodes a member of the calnexin family of molecular chaperones. The encoded protein is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq]
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Zhang C, Chong X, Jiang F, et al. Plasma extracellular vesicle derived protein profile predicting and monitoring immunotherapeutic outcomes of gastric cancer. J Extracell Vesicles. 2022,11(4):e12209. doi: 10.1002/jev2.12209
    Read this paper
  • Khan AA, Man F, Faruqu FN, et al. PET Imaging of Small Extracellular Vesicles via [(89)Zr]Zr(oxinate)(4) Direct Radiolabeling. Bioconjug Chem. 2022,33(3):473-485. doi: 10.1021/acs.bioconjchem.1c00597
    Read this paper
  • Gao Z, Zhang L, Ma J, et al. Development of antibody-based assays for high throughput discovery and mechanistic study of antiviral agents against yellow fever virus. Antiviral Res. 2020,182:104907. doi: 10.1016/j.antiviral.2020.104907
    Read this paper
  • Sikorski K, Mehta A, Inngjerdingen M, et al. A high-throughput pipeline for validation of antibodies. Nat Methods. 2018,15(11):909-912. doi: 10.1038/s41592-018-0179-8
    Read this paper

Datasheet

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Figure 1. Western blot analysis of Calnexin/CANX using anti-Calnexin/CANX antibody (A03372-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human U-937 whole cell lysates, Lane 3: human placenta tissue lysates, Lane 4: human THP-1 whole cell lysates, Lane 5: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Calnexin/CANX antigen affinity purified polyclonal antibody (Catalog # A03372-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Calnexin/CANX at approximately 97 kDa. The expected band size for Calnexin/CANX is at 97 kDa.
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