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ProductsBack/Calpain 2 antibody [1E8]

Calpain 2 antibody [1E8]

GTX20713
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
DownloadDatasheet
Product group Antibodies
TargetCAPN2
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Overview

  • Supplier
    GeneTex
  • Product Name
    Calpain 2 antibody [1E8]
  • Delivery Days Customer
    9
  • Application Supplier Note
    Western blot: 1 microg/ml. Immunofluorescence: use the antibody after 2% paraformaldehyde fixation in a concentration of 0.5 microg/ml.
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Concentration
    2 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID824
  • Target name
    CAPN2
  • Target description
    calpain 2
  • Target synonyms
    calcium-activated neutral proteinase 2; calpain 2, (m/II) large subunit; calpain 2, large [catalytic] subunit; calpain 2, large subunit; calpain M-type; calpain, large polypeptide L2; calpain-2 catalytic subunit; CANP 2; CANP2; CANPL2; CANPml; M-calpain; mCANP; millimolar-calpain
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP17655
  • Protein Name
    Calpain-2 catalytic subunit
  • Scientific Description
    The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 2. Multiple heterogeneous transcriptional start sites in the 5 UTR have been reported. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Calpain 2 using anti-Calpain 2 antibody (A03492). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: rat kidney tissue lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse kidney tissue lysates, Lane 7: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Calpain 2 antigen affinity purified polyclonal antibody (Catalog # A03492) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Calpain 2 at approximately 80 kDa. The expected band size for Calpain 2 is at 80 kDa.
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