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Immunohistochemistry of paraffin-embedded Human liver damage using CARD10 Polyclonal Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Human liver damage using CARD10 Polyclonal Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Human liver damage using CARD10 Polyclonal Antibody at dilution of 1:100 (40x lens).

CARD10 Polyclonal Antibody

E-AB-61673
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    CARD10 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Certification
    Research Use Only
  • Scientific Description
    The caspase recruitment domain (CARD) is a protein module that consists of 6 or 7 antiparallel alpha helices. It participates in apoptosis signaling through highly specific protein-protein homophilic interactions. Like several other CARD proteins, CARD10 belongs to the membrane-associated guanylate kinase (MAGUK) family and activates NF-kappa-B through BCL10.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CARD10 using anti-CARD10 antibody (A07686-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human Hacat whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CARD10 antigen affinity purified polyclonal antibody (A07686-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CARD10 at approximately 116 kDa. The expected band size for CARD10 is at 116 kDa.
Product group Antibodies
Boster Bio
Anti-CARD10 Antibody Picoband(r)A07686-4-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetCARD10
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Product group Antibodies
RayBiotech
Anti-CARD10 Antibody144-66373
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse
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Western Blot analysis of 293 cells using CARD 10 Polyclonal Antibody
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ReactivityHuman, Mouse
TargetCARD10
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Product group Antibodies
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Immunofluorescent staining of human cell line HeLa shows localization to vesicles.
Product group Antibodies
Atlas Antibodies
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TargetCARD10
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Product group Antibodies
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Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CARD10 antibody [N2C1], Internal (GTX111222) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Product group Antibodies
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GeneTex
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Anti-CARD10 AntibodyA97094
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