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ProductsBack/Cardiac Troponin I antibody [4A3]
WB analysis of HEK293T cells transfected with Cardiac Troponin I plasmid (Right) or empty vector (Left) for 48 hrs using GTX83501 Cardiac Troponin I antibody [4A3]. Loading : 5 ug per lane
WB analysis of HEK293T cells transfected with Cardiac Troponin I plasmid (Right) or empty vector (Left) for 48 hrs using GTX83501 Cardiac Troponin I antibody [4A3]. Loading : 5 ug per lane
WB analysis of HEK293T cells transfected with Cardiac Troponin I plasmid (Right) or empty vector (Left) for 48 hrs using GTX83501 Cardiac Troponin I antibody [4A3]. Loading : 5 ug per lane

Cardiac Troponin I antibody [4A3]

GTX83501
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetTNNI3
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Overview

  • Supplier
    GeneTex
  • Product Name
    Cardiac Troponin I antibody [4A3]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:2000. IHC-P: 1:50. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    4A3
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID7137
  • Target name
    TNNI3
  • Target description
    troponin I3, cardiac type
  • Target synonyms
    CMD1FF, CMD2A, CMH7, RCM1, TNNC1, cTnI, troponin I, cardiac muscle, cardiomyopathy, dilated 2A (autosomal recessive), troponin I type 3 (cardiac)
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP19429
  • Protein Name
    Troponin I, cardiac muscle
  • Scientific Description
    Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Cardiac Troponin I/TNNI3 using anti-Cardiac Troponin I/TNNI3 antibody (A01720-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cardiac Troponin I/TNNI3 antigen affinity purified polyclonal antibody (Catalog # A01720-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cardiac Troponin I/TNNI3 at approximately 24 kDa. The expected band size for Cardiac Troponin I/TNNI3 is at 24 kDa.
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