![Rat tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Cardiac Troponin I antibody [N1C3-2] (GTX112770) diluted at 1:10000. Rat tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Cardiac Troponin I antibody [N1C3-2] (GTX112770) diluted at 1:10000.](https://www.genetex.com/upload/website/prouct_img/normal/GTX112770/GTX112770_40079_20160121_WB_R_heart_w_23060500_756.webp)
Rat tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Cardiac Troponin I antibody [N1C3-2] (GTX112770) diluted at 1:10000.
Cardiac Troponin I antibody [N1C3-2]
GTX112770
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetTNNI3
Overview
- SupplierGeneTex
- Product NameCardiac Troponin I antibody [N1C3-2]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:1000-1:20000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID7137
- Target nameTNNI3
- Target descriptiontroponin I3, cardiac type
- Target synonymsCMD1FF, CMD2A, CMH7, RCM1, TNNC1, cTnI, troponin I, cardiac muscle, cardiomyopathy, dilated 2A (autosomal recessive), troponin I type 3 (cardiac)
- HostRabbit
- IsotypeIgG
- Protein IDP19429
- Protein NameTroponin I, cardiac muscle
- Scientific DescriptionTroponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM). [provided by RefSeq]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
![Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Cardiac Troponin I antibody [N1C3-2] (GTX112770) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Cardiac Troponin I antibody [N1C3-2] (GTX112770) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX112770/GTX112770_40079_20240531_WB_M_heart_24060619_607.webp)


![Cardiac Troponin I antibody [N1C3] detects Cardiac Troponin I protein at cytosol on mouse heart by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. Cardiac Troponin I antibody [N1C3] (GTX109331) dilution: 1:500. Scale bar = M μm.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min](https://www.genetex.com/upload/website/prouct_img/normal/GTX109331/GTX109331_40100_IHC_M_w_23060500_480.webp)
![Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Cardiac Troponin I antibody [HL1489] (GTX636963) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX636963/GTX636963_44732_20220715_WB_M_tissue_22071823_668.webp)

![WB analysis of HEK293T cells transfected with Cardiac Troponin I plasmid (Right) or empty vector (Left) for 48 hrs using GTX83501 Cardiac Troponin I antibody [4A3]. Loading : 5 ug per lane](https://www.genetex.com/upload/website/prouct_img/normal/GTX83501/GTX83501_3676_WB_w_23061419_256.webp)