More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/Caspase 3 antibody [HL1370]
Wild-type (WT) and Caspase 3 knockout (KO) HeLa cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Caspase 3 antibody [HL1370] (GTX636810) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Wild-type (WT) and Caspase 3 knockout (KO) HeLa cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Caspase 3 antibody [HL1370] (GTX636810) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Wild-type (WT) and Caspase 3 knockout (KO) HeLa cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Caspase 3 antibody [HL1370] (GTX636810) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Caspase 3 antibody [HL1370]

GTX636810
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Rat
TargetCASP3
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    GeneTex
  • Product Name
    Caspase 3 antibody [HL1370]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    HL1370
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID836
  • Target name
    CASP3
  • Target description
    caspase 3
  • Target synonyms
    CPP32, CPP32B, SCA-1, caspase-3, CASP-3, CPP-32, PARP cleavage protease, SREBP cleavage activity 1, apopain, caspase 3, apoptosis-related cysteine peptidase, caspase 3, apoptosis-related cysteine protease, cysteine protease CPP32, procaspase3, protein Yama
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP42574
  • Protein Name
    Caspase-3
  • Scientific Description
    The protein encoded by this gene is a cysteine-aspartic acid protease that plays a central role in the execution-phase of cell apoptosis. The encoded protein cleaves and inactivates poly(ADP-ribose) polymerase while it cleaves and activates sterol regulatory element binding proteins as well as caspases 6, 7, and 9. This protein itself is processed by caspases 8, 9, and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimers disease. [provided by RefSeq, Aug 2017]
  • Reactivity
    Human, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

Datasheet

Download Datasheet

FAQ

Go to our frequently asked questions

Related products

Product group Antibodies
Absolute Antibody
Anti-Caspase-3 [4F-6]AB04219-1.1
ApplicationsWestern Blot, ELISA, Neutralisation/Blocking
ReactivityHuman
TargetCASP3
  • SizePrice
Product group Antibodies
Biosynth
CASP3 antibody70R-16179
  • SizePrice
Product group Antibodies
RayBiotech
Anti-CASP3 (138-157aa) Antibody130-10514
ApplicationsELISA
ReactivityHuman
TargetCASP3
  • SizePrice
Figure 1. Western blot analysis of Caspase-3(P17)/CASP3 using anti-Caspase-3(P17)/CASP3 antibody (A00334-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Caspase-3(P17)/CASP3 antigen affinity purified polyclonal antibody (Catalog # A00334-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Caspase-3(P17)/CASP3 at approximately 32 kDa. The expected band size for Caspase-3(P17)/CASP3 is at 32 kDa.
Product group Antibodies
Boster Bio
Anti-Caspase-3(p17)/CASP3 Antibody Picoband(r)A00334-2-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman
TargetCASP3
  • SizePrice
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Histone H2A antibody (GTX123678) diluted at 1:200. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Product group Antibodies
References
GeneTex
Caspase 3 antibodyGTX123678
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Insect, Mouse, Rat
TargetCASP3
  • SizePrice
IHC-P analysis of human ehrlich carcimoma tissue using GTX03281 Caspase 3 (cleaved Asp175) antibody. Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed. Dilution : 1:100
Product group Antibodies
References
GeneTex
Caspase 3 (cleaved Asp175) antibodyGTX03281
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse
TargetCASP3
  • SizePrice
IHC-P analysis of rat lung tissue using GTX04908 Caspase 3 antibody. Antigen retrieval : Heat mediated citrate buffer Dilution : 1:100
Product group Antibodies
GeneTex
Caspase 3 antibodyGTX04908
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityBovine, Human, Mouse, Rat
TargetCASP3
  • SizePrice
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Caspase 3 antibody (GTX110543) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Product group Antibodies
References
GeneTex
Caspase 3 antibodyGTX110543
ApplicationsDot Blot, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Rat
TargetCASP3
  • SizePrice
WB analysis of HeLa cells treated with camptothecin (2μM) lysate using GTX59551 Caspase 3 antibody [C33].
Product group Antibodies
References
GeneTex
Caspase 3 antibody [C33]GTX59551
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetCASP3
  • SizePrice
The data was published in the journal Evid Based Complement Alternat Med in 2012.
Product group Antibodies
References
GeneTex
Caspase 3 antibodyGTX73090
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Rabbit, Rat
TargetCASP3
  • SizePrice