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ProductsBack/CCL3 / MIP-1-Alpha Antibody (aa24-92)

CCL3 / MIP-1-Alpha Antibody (aa24-92)

LS-C407783
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetCCL3
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    CCL3 / MIP-1-Alpha Antibody (aa24-92)
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA (0.1 - 0.5 µg/ml), WB (0.1 - 0.5 µg/ml)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID6348
  • Target name
    CCL3
  • Target description
    C-C motif chemokine ligand 3
  • Target synonyms
    G0S19-1, LD78, LD78ALPHA, MIP-1-alpha, MIP1A, SCI, SCYA3, C-C motif chemokine 3, G0/G1 switch regulatory protein 19-1, PAT 464.1, SIS-beta, macrophage inflammatory protein 1-alpha, small inducible cytokine A3 (homologous to mouse Mip-1a), tonsillar lymphocyte LD78 alpha protein
  • Host
    Rabbit
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CCL3 using anti-CCL3 antibody (PB9571). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: JURKAT Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCL3 antigen affinity purified polyclonal antibody (Catalog # PB9571) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCL3 at approximately 11 kDa. The expected band size for CCL3 is at 11 kDa.
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