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ProductsBack/CD11b antibody [M1/70.15] (PE)
Staining of total mouse peritoneal exudate cells demonstrating labelling of macrophages with Rat anti Mouse CD11b:RPE (GTX76475)
Staining of total mouse peritoneal exudate cells demonstrating labelling of macrophages with Rat anti Mouse CD11b:RPE (GTX76475)
Staining of total mouse peritoneal exudate cells demonstrating labelling of macrophages with Rat anti Mouse CD11b:RPE (GTX76475)

CD11b antibody [M1/70.15] (PE)

GTX76470
GeneTex
ApplicationsFlow Cytometry
Product group Antibodies
ReactivityMouse
TargetItgam
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Overview

  • Supplier
    GeneTex
  • Product Name
    CD11b antibody [M1/70.15] (PE)
  • Delivery Days Customer
    9
  • Application Supplier Note
    For FACS: Use 0.1 ug to label 10^6 cells. Protocols are available upon request. Optimal dilutions/concentrations should be determined by the end user.
  • Applications
    Flow Cytometry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    M1/70.15
  • Concentration
    0.1 mg/ml
  • Conjugate
    RPE
  • Gene ID16409
  • Target name
    Itgam
  • Target description
    integrin alpha M
  • Target synonyms
    CD11b/CD18, CR3, CR3A, Cd11b, F730045J24Rik, Ly-40, MAC1, Mac-1, Mac-1a, integrin alpha-M, CD11 antigen-like family member B, CD11B (p170), CR-3 alpha chain, Mac-1 alpha, cell surface glycoprotein MAC-1 alpha subunit, cell surface glycoprotein MAC-1 subunit alpha, complement receptor type 3, leukocyte adhesion receptor MO1, macrophage antigen alpha
  • Host
    Rat
  • Isotype
    IgG2b
  • Scientific Description
    Recognises the murine CD11b cell surface antigen (also known as the alpha M integrin chain), a differentiation antigen expressed by granulocytes, monocytes, NK cells and tissue macrophages. The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages. Peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages. Clone M1/70.15 has been reported to block iC3b binding to its receptor (3).
  • Reactivity
    Mouse
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CD11B/Integrin Alpha M/Itgam using anti-CD11B/Integrin Alpha M/Itgam antibody (A00144-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse ANA-1 whole cell lysates, Lane 2: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD11B/Integrin Alpha M/Itgam antigen affinity purified polyclonal antibody (Catalog # A00144-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD11B/Integrin Alpha M/Itgam at approximately 170 kDa. The expected band size for CD11B/Integrin Alpha M/Itgam is at 170 kDa.
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