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ProductsBack/CD11b antibody [M1/70.15] (PE)

CD11b antibody [M1/70.15] (PE)

GTX76475
GeneTex
ApplicationsFlow Cytometry
Product group Antibodies
TargetItgam
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Overview

  • Supplier
    GeneTex
  • Product Name
    CD11b antibody [M1/70.15] (PE)
  • Delivery Days Customer
    9
  • Application Supplier Note
    FACS: Neat - 1/10. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    M1/70.15
  • Conjugate
    RPE
  • Gene ID16409
  • Target name
    Itgam
  • Target description
    integrin alpha M
  • Target synonyms
    CD11 antigen-like family member B; Cd11b; CD11B (p170); CD11b/CD18; cell surface glycoprotein MAC-1 alpha subunit; cell surface glycoprotein MAC-1 subunit alpha; complement receptor type 3; CR; CR3; CR-3 alpha chain; CR3A; F730045J24Rik; integrin alpha-M; leukocyte adhesion receptor MO1; Ly-4; Ly-40; Mac-; MAC1; Mac-1; Mac-1 alpha; Mac-1a; macrophage antigen alpha
  • Host
    Rat
  • Isotype
    IgG2b
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

References

  • Extracellular DJ-1 induces sterile inflammation in the ischemic brain. Nakamura K et al., 2021 May, PLoS Biol
    Read more
  • Characteristics of fucose-containing polysaccharides from submerged fermentation of Agaricus blazei Murill.
    Read more
  • Trajectory of inflammatory and microglial activation markers in the postnatal rabbit brain following intrauterine endotoxin exposure. Zhang Z et al., 2018 Mar, Neurobiol Dis
    Read more
  • A Type II Arabinogalactan from Anoectochilus formosanus for G-CSF Production in Macrophages and Leukopenia Improvement in CT26-Bearing Mice Treated with 5-Fluorouracil. Yang LC et al., 2013, Evid Based Complement Alternat Med
    Read more

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Figure 1. Western blot analysis of CD11B/Integrin Alpha M/Itgam using anti-CD11B/Integrin Alpha M/Itgam antibody (A00144-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse ANA-1 whole cell lysates, Lane 2: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD11B/Integrin Alpha M/Itgam antigen affinity purified polyclonal antibody (Catalog # A00144-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD11B/Integrin Alpha M/Itgam at approximately 170 kDa. The expected band size for CD11B/Integrin Alpha M/Itgam is at 170 kDa.
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