![CD11b antibody [HL3095] detects CD11b protein by immunofluorescent analysis. Sample: Raw264.7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CD11b stained by CD11b antibody [HL3095] (GTX640541) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920). CD11b antibody [HL3095] detects CD11b protein by immunofluorescent analysis. Sample: Raw264.7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CD11b stained by CD11b antibody [HL3095] (GTX640541) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX640541/GTX640541_T-45453_20240816_ICC_IF_M_24082301_339.webp)
CD11b antibody [HL3095] detects CD11b protein by immunofluorescent analysis. Sample: Raw264.7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CD11b stained by CD11b antibody [HL3095] (GTX640541) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).
CD11b antibody [HL3095]
GTX640541
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityMouse
TargetItgam
Overview
- SupplierGeneTex
- Product NameCD11b antibody [HL3095]
- Delivery Days Customer7
- Application Supplier NoteICC/IF: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL3095
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID16409
- Target nameItgam
- Target descriptionintegrin alpha M
- Target synonymsCD11b/CD18, CR3, CR3A, Cd11b, F730045J24Rik, Ly-40, MAC1, Mac-1, Mac-1a, integrin alpha-M, CD11 antigen-like family member B, CD11B (p170), CR-3 alpha chain, Mac-1 alpha, cell surface glycoprotein MAC-1 alpha subunit, cell surface glycoprotein MAC-1 subunit alpha, complement receptor type 3, leukocyte adhesion receptor MO1, macrophage antigen alpha
- HostRabbit
- IsotypeIgG
- Protein IDP05555
- Protein NameIntegrin alpha-M
- Scientific DescriptionEnables complement component C3b binding activity; heparan sulfate proteoglycan binding activity; and heparin binding activity. Contributes to cargo receptor activity. Involved in several processes, including central nervous system development; endocytosis; and positive regulation of neuron death. Acts upstream of or within several processes, including activated T cell proliferation; leukocyte migration; and microglia development. Located in external side of plasma membrane and nucleus. Is integral component of membrane. Part of integrin alphaM-beta2 complex. Is expressed in several structures, including central nervous system; heart; lymphatic vessel; thymus; and yolk sac. Human ortholog(s) of this gene implicated in lupus nephritis. Orthologous to human ITGAM (integrin subunit alpha M). [provided by Alliance of Genome Resources, Apr 2022]
- ReactivityMouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
![Boiled and unboiled various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with CD11b antibody [HL3095] (GTX640541) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Boiled and unboiled various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with CD11b antibody [HL3095] (GTX640541) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX640541/GTX640541_T-45453_20240823_WB_M_tissue_ub_24082802_495.webp)


![IHC-Fr analysis of mouse lymph node using CD11b antibody [5C6] (green), CD8 antibody [YTS105.18] (red, GTX74642) and DAPI (blue).](https://www.genetex.com/upload/website/prouct_img/normal/GTX42476/GTX42476_277_IHC-Fr_w_23060820_428.webp)
![CD11b antibody [HL3097] detects CD11b protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Green: CD11b stained by CD11b antibody [HL3097] (GTX640543) diluted at 1:250. Blue: Hoechst 33342 staining. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX640543/GTX640543_T-45453_20240802_IHC-P_M_24081300_176.webp)