CD11b antibody [HL3097] detects CD11b protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Green: CD11b stained by CD11b antibody [HL3097] (GTX640543) diluted at 1:250. Blue: Hoechst 33342 staining. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
![CD11b antibody [HL3097] detects CD11b protein by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Green: CD11b stained by CD11b antibody [HL3097] (GTX640543) diluted at 1:250. Blue: Hoechst 33342 staining. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX640543/GTX640543_T-45453_20240802_IHC-P_R_24081300_924.webp "CD11b antibody [HL3097] detects CD11b protein by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Green: CD11b stained by CD11b antibody [HL3097] (GTX640543) diluted at 1:250. Blue: Hoechst 33342 staining. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Boiled and unboiled Raw 264.7 whole cell and membrane extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with CD11b antibody [HL3097] (GTX640543) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract)](https://www.genetex.com/upload/website/prouct_img/normal/GTX640543/GTX640543_45509_20240823_WB_M_Fraction_ub_24090423_767.webp "Boiled and unboiled Raw 264.7 whole cell and membrane extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with CD11b antibody [HL3097] (GTX640543) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract)")
![Rat tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with CD11b antibody [HL3097] (GTX640543) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX640543/GTX640543_45509_20250523_WB_R_spleen_25052800_387.webp "Rat tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with CD11b antibody [HL3097] (GTX640543) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
CD11b antibody [HL3097] detects CD11b protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Green: CD11b stained by CD11b antibody [HL3097] (GTX640543) diluted at 1:250. Blue: Hoechst 33342 staining. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
CD11b antibody [HL3097]
GTX640543
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityMouse, Rat
TargetItgam
Overview
- SupplierGeneTex
- Product NameCD11b antibody [HL3097]
- Delivery Days Customer7
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL3097
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID16409
- Target nameItgam
- Target descriptionintegrin alpha M
- Target synonymsCD11b/CD18, CR3, CR3A, Cd11b, F730045J24Rik, Ly-40, MAC1, Mac-1, Mac-1a, integrin alpha-M, CD11 antigen-like family member B, CD11B (p170), CR-3 alpha chain, Mac-1 alpha, cell surface glycoprotein MAC-1 alpha subunit, cell surface glycoprotein MAC-1 subunit alpha, complement receptor type 3, leukocyte adhesion receptor MO1, macrophage antigen alpha
- HostRabbit
- IsotypeIgG
- Protein IDP05555
- Protein NameIntegrin alpha-M
- Scientific DescriptionEnables complement component C3b binding activity; heparan sulfate proteoglycan binding activity; and heparin binding activity. Contributes to cargo receptor activity. Involved in several processes, including central nervous system development; endocytosis; and positive regulation of neuron death. Acts upstream of or within several processes, including activated T cell proliferation; leukocyte migration; and microglia development. Located in external side of plasma membrane and nucleus. Is integral component of membrane. Part of integrin alphaM-beta2 complex. Is expressed in several structures, including central nervous system; heart; lymphatic vessel; thymus; and yolk sac. Human ortholog(s) of this gene implicated in lupus nephritis. Orthologous to human ITGAM (integrin subunit alpha M). [provided by Alliance of Genome Resources, Apr 2022]
- ReactivityMouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
Datasheet
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