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CD137 Recombinant Antibody AE00312

AE00312
Aeonian Biotech
TargetTnfrsf9
Product group Antibodies
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Overview

  • Supplier
    Aeonian Biotech
  • Product Name
    CD137 Recombinant Antibody AE00312
  • Delivery Days Customer
    9
  • Applications Supplier
    FC, CD8+ T cell activation
  • Certification
    Research Use Only
  • Clonality
    Recombinant
  • Clone ID
    rLOB12.3
  • Conjugate
    Unconjugated
  • Gene ID21942
  • Target name
    Tnfrsf9
  • Target description
    tumor necrosis factor receptor superfamily, member 9
  • Target synonyms
    4-1BB, A930040I11Rik, CDw137, Cd137, ILA, Ly63, tumor necrosis factor receptor superfamily member 9, 4-1BB ligand receptor, CD137 antigen, T-cell antigen 4-1BB, secreted CD137 antigen
  • Host
    Rat
  • Isotype
    IgG1 kappa
  • Protein IDP20334
  • Protein Name
    Tumor necrosis factor receptor superfamily member 9
  • Scientific Description
    CD137 Recombinant Antibody AE00312
  • Shelf life instruction
    Integrity warranted for 24 months after purchase when handled and stored according to instructions, see below.
  • Reactivity Supplier
    Mouse
  • Storage Instruction
    2-8°C
  • UNSPSC
    12352203

Datasheet

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MSDS

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FAQ

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Figure 1. Western blot analysis of CD137 using anti-CD137 antibody (A00867-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant mouse CD137 protein 1ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD137 antigen affinity purified polyclonal antibody (Catalog # A00867-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD137 at approximately 65KD. The expected band size for CD137 is at 44KD.
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