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ProductsBack/CD274 / B7-H1 / PD-L1 Antibody (clone R639, Preservative Free)

CD274 / B7-H1 / PD-L1 Antibody (clone R639, Preservative Free)

LS-C819125
LifeSpan BioSciences
ApplicationsELISA, Neutralisation/Blocking
Product group Antibodies
TargetCD274
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    CD274 / B7-H1 / PD-L1 Antibody (clone R639, Preservative Free)
  • Delivery Days Customer
    14
  • Application Supplier Note
    Block: In a functional ELISA which immobilized recombinant Human PD1 /Fc Chimera at 10ug/ml (100 microL/well) in the plate, the Rabbit Anti-Human PD-L1 Monoclonal Antibody can block the binding of 0.1 ug/ml of recombinant Human PD-L1 to human PD1, the EC50 is 0.83 ug/ml. Please Note: Optimal concentrations/dilutions should be determined by the end user.. Block (0.1 µg/ml), ELISA (10 µg/ml), Neut Block: In a functional ELISA which immobilized recombinant Human PD1 /Fc Chimera at 10ug/ml (100 µL/well) in the plate, the Rabbit Anti-Human PD-L1 Monoclonal Antibody can block the binding of 0.1 ug/ml of recombinant Human PD-L1 to human PD1, the EC50 is 0.83 ug/ml. Please Note: Optimal concentrations/dilutions should be determined by the end user.
  • Applications
    ELISA, Neutralisation/Blocking
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    R639
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID29126
  • Target name
    CD274
  • Target description
    CD274 molecule
  • Target synonyms
    ADMIO5, B7-H, B7H1, PD-L1, PDCD1L1, PDCD1LG1, PDL1, hPD-L1, programmed cell death 1 ligand 1, B7 homolog 1, CD274 antigen, PDCD1 ligand 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PD-L1/B7-H1 using anti-PD-L1/B7-H1 antibody (A00109). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: HELA whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PD-L1/B7-H1 antigen affinity purified polyclonal antibody (Catalog # A00109) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PD-L1/B7-H1 at approximately 62KD. The expected band size for PD-L1/B7-H1 is at 33KD.
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