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CD300A Antibody

LS-C813805
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetCD300A
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    CD300A Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA (1:10000 - 1:20000), WB (1:500 - 1:2000) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID11314
  • Target name
    CD300A
  • Target description
    CD300a molecule
  • Target synonyms
    CD300 antigen-like family member A; CD300a antigen; CLM-8; CMRF35H; CMRF-35H; CMRF35-H; CMRF35H leukocyte immunoglobulin-like receptor; CMRF35H9; CMRF-35-H9; CMRF35-H9; CMRF35-like molecule 8; IGSF12; immunoglobulin superfamily member 12; inhibitory receptor protein 60; IRC1; IRC1/IRC2; IRC2; IRp60; leukocyte membrane antigen; NK inhibitory receptor
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD300A using anti-CD300A antibody (A05953-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: rat PC-12 whole cell lysates, Lane 4: rat C6 whole cell lysates, Lane 5: mouse Raw264.7 whole cell lysates, Lane 6: mouse J774A.1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD300A antigen affinity purified polyclonal antibody (Catalog # A05953-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD300A at approximately 68 kDa. The expected band size for CD300A is at 33 kDa.
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