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CD314/NKG2D Antibody (YA3918, Mouse)

HY-P84221
MedChem Express
TargetKLRK1
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    CD314/NKG2D Antibody (YA3918, Mouse)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID22914
  • Target name
    KLRK1
  • Target description
    killer cell lectin like receptor K1
  • Target synonyms
    CD314, D12S2489E, KLR, NKG2-D, NKG2D, NKG2-D type II integral membrane protein, NK cell receptor D, NKG2-D-activating NK receptor, killer cell lectin-like receptor subfamily K, member 1
  • Host
    Mouse
  • Isotype
    IgG2a
  • Scientific Description
    CD314/NKG2D Antibody (YA3918) is a mouse-derived and non-conjugated IgG2a antibody, targeting to CD314. It can be applicated for WB, FC, ELISA assays, in the background of human, rat.
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of NKG2D using anti-NKG2D antibody (A00661-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat lymph tissue lysates, Lane 2: rat spleen tissue lysates, Lane 3: mouse thymus tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NKG2D antigen affinity purified polyclonal antibody (Catalog # A00661-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NKG2D at approximately 35KD. The expected band size for NKG2D is at 25KD.
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