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CD36 Antibody

LS-C403039
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetCD36
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    CD36 Antibody
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA, IHC (1:50 - 1:200), WB (1:500 - 1:2000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.8 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID948
  • Target name
    CD36
  • Target description
    CD36 molecule (CD36 blood group)
  • Target synonyms
    BDPLT10, CHDS7, FAT, GP3B, GP4, GPIV, PASIV, SCARB3, platelet glycoprotein 4, CD36 antigen (collagen type I receptor, thrombospondin receptor), CD36 molecule (CD36 blood group) transcript, CD36 molecule (thrombospondin receptor), GPIIIB, PAS IV, PAS-4 protein, cluster determinant 36, fatty acid translocase, glycoprotein IIIb, leukocyte differentiation antigen CD36, platelet glycoprotein IV, scavenger receptor class B, member 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CD36 using anti-CD36 antibody (A01189-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, Lane 2: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD36 antigen affinity purified polyclonal antibody (Catalog # A01189-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD36 at approximately 88 kDa. The expected band size for CD36 is at 53 kDa.
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Untreated (–) and treated (+) THP-1 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD36 antibody [C1C3] (GTX100642) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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