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Western Blot Positive WB detected in: Rat Liver tissue lysate, Rat Lung tissue lysate, Mouse Liver tissue lysate, Mouse Lung tissue lysate All lanes: CD9 antibody at 1:500 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 25 kDa Observed band size: 25 kDa
Western Blot Positive WB detected in: Rat Liver tissue lysate, Rat Lung tissue lysate, Mouse Liver tissue lysate, Mouse Lung tissue lysate All lanes: CD9 antibody at 1:500 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 25 kDa Observed band size: 25 kDa
Western Blot Positive WB detected in: Rat Liver tissue lysate, Rat Lung tissue lysate, Mouse Liver tissue lysate, Mouse Lung tissue lysate All lanes: CD9 antibody at 1:500 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 25 kDa Observed band size: 25 kDa

CD9 Antibody

CSB-PA10559A0RB
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCD9
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Overview

  • Supplier
    Cusabio
  • Product Name
    CD9 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID928
  • Target name
    CD9
  • Target description
    CD9 molecule
  • Target synonyms
    BTCC-1, DRAP-27, MIC3, MRP-1, TSPAN-29, TSPAN29, CD9 antigen, 5H9 antigen, BA-2/p24 antigen, CD9 antigen (p24), antigen CD9, cell growth-inhibiting gene 2 protein, leukocyte antigen MIC3, motility related protein-1, tetraspanin-29
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP21926
  • Protein Name
    CD9 antigen
  • Scientific Description
    Involved in platelet activation and aggregation. Regulates paranodal junction formation. Involved in cell adhesion, cell motility and tumor metastasis. Required for sperm-egg fusion.
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CD9 using anti-CD9 antibody (A01202-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HCCT tissue lysates, Lane 2: human RT4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD9 antigen affinity purified polyclonal antibody (Catalog # A01202-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD9 at approximately 22 kDa. The expected band size for CD9 is at 25 kDa.
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