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Western Blot Positive WB detected in U87 whole cell lysate All lanes CD9 antibody at 0.55microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 25 KDa Observed band size: 25 KDa

IHC image of CSB-RA004969A0HU diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of MCF-7 cells with CSB-RA004969A0HU at 1:34,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Overlay histogram showing Jurkat cells stained with CSB-RA004969A0HU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min.The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C.The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4°C. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.

CD9 Antibody is validated for ELISA, FC, IF, IHC, WB & tested in Human. Cusabio is available at Bio-Connect

CD9 Antibody antibody | CSB-RA004969A0HU | Cusabio

CD9 Recombinant Monoclonal Antibody

Antibodies

Involved in platelet activation and aggregation. Regulates paranodal junction formation. Involved in cell adhesion, cell motility and tumor metastasis. Required for sperm-egg fusion.

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CD9 Recombinant Monoclonal Antibody

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