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Cdc25B (Phospho-Ser149) Antibody

MBS9414074
MyBioSource
Product group Antibodies
Price on request
0.05 ml
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More than 130 suppliers
ISO certified
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Overview

  • Supplier
    MyBioSource
  • Product Name
    Cdc25B (Phospho-Ser149) Antibody
  • Delivery Days Customer
    16
  • Certification
    Research Use Only
  • Concentration
    1 mg/ml
  • Storage Instruction
    2°C to 8°C,-20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Cdc25b using anti-Cdc25b antibody (A01899). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cdc25b antigen affinity purified polyclonal antibody (Catalog # A01899) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cdc25b at approximately 65 kDa. The expected band size for Cdc25b is at 65 kDa.
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