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ProductsBack/CDK2 Antibody (aa81-298)

CDK2 Antibody (aa81-298)

LS-C407747
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCDK2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    CDK2 Antibody (aa81-298)
  • Delivery Days Customer
    23
  • Application Supplier Note
    IHC: Antigen retrieval by boiling the paraffin sections in 10 mM citrate buffer, pH6.0, for 20 minutes is required for the staining of formalin/paraffin sections.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC, IHC-P (0.5 - 1 µg/ml), WB (0.1 - 0.5 µg/ml) IHC: Antigen retrieval by boiling the paraffin sections in 10 mM citrate buffer, pH6.0, for 20 minutes is required for the staining of formalin/paraffin sections.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID1017
  • Target name
    CDK2
  • Target description
    cyclin dependent kinase 2
  • Target synonyms
    CDKN2, p33(CDK2), cyclin-dependent kinase 2, cdc2-related protein kinase, cell division protein kinase 2, p33 protein kinase
  • Host
    Rabbit
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Cdk2 using anti-Cdk2 antibody (PB9534). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurlat whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human U2OS whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: human T47D whole cell lysates, Lane 6: human CACO-2 whole cell lysates, Lane 7: human 293T whole cell lysates, Lane 8: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cdk2 antigen affinity purified polyclonal antibody (Catalog # PB9534) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cdk2 at approximately 30 kDa. The expected band size for Cdk2 is at 30, 33 kDa.
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