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ProductsBack/CDK6 Mouse Monoclonal Antibody [Clone ID: DCS-83.1]

CDK6 Mouse Monoclonal Antibody [Clone ID: DCS-83.1]

AM05226PU-N
OriGene
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetCDK6
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    CDK6 Mouse Monoclonal Antibody [Clone ID: DCS-83.1]
  • Delivery Days Customer
    14
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    DCS-83.1
  • Gene ID1021
  • Target name
    CDK6
  • Target description
    cyclin dependent kinase 6
  • Target synonyms
    MCPH12, PLSTIRE, cyclin-dependent kinase 6, cell division protein kinase 6, serine/threonine-protein kinase PLSTIRE
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ00534
  • Protein Name
    Cyclin-dependent kinase 6
  • Scientific Description
    CDK6 mouse monoclonal antibody, clone DCS-83.1, Purified
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Cdk6 using anti-Cdk6 antibody (A00358). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cdk6 antigen affinity purified polyclonal antibody (Catalog # A00358) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cdk6 at approximately 37 kDa. The expected band size for Cdk6 is at 37 kDa.
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