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CDK7 Antibody

LS-C816091
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetCDK7
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    CDK7 Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ICC (1:100 - 1:500), IF (1:100 - 1:500), IHC-P (1:50 - 1:200), WB (1:500 - 1:2000) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID1022
  • Target name
    CDK7
  • Target description
    cyclin dependent kinase 7
  • Target synonyms
    CAK, CAK1, CDKN7, HCAK, MO15, STK1, p39MO15, cyclin-dependent kinase 7, 39 KDa protein kinase, CDK-activating kinase 1, TFIIH basal transcription factor complex kinase subunit, cell division protein kinase 7, cyclin-dependent kinase 7 (MO15 homolog, Xenopus laevis, cdk-activating kinase), homolog of Xenopus MO15 Cdk-activating kinase, kinase subunit of CAK, serine/threonine kinase stk1, serine/threonine protein kinase 1, serine/threonine protein kinase MO15
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

FAQ

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Figure 1. Western blot analysis of Cdk7 using anti-Cdk7 antibody (PB9536). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: MCF-7 Whole Cell Lysate at 40ug, Lane 2: HELA Whole Cell Lysate at 40ug, Lane 3: A549 Whole Cell Lysate at 40ug, Lane 4: HEPG2 Whole Cell Lysate at 40ug, Lane 5: 293T Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cdk7 antigen affinity purified polyclonal antibody (Catalog # PB9536) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cdk7 at approximately 39 kDa. The expected band size for Cdk7 is at 39 kDa.
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