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ProductsBack/CENPF antibody [14C10 1D8]
Confocal immunofluorescence staining (Olympus FV10i) of Mitosin. U2OS cells were fixed by 4% PFA and costained with Mitosin 14C10 1D8 AB (Red; cat# GTX70137; 1:500 Ab dilution) and alpha-tubulin rabbit polyclonal AB (Green; cat# GTX112141; 1:500 Ab dilution ), a spindle marker. DAPI (blue), chromosomes. Scale bar, 5 um
Confocal immunofluorescence staining (Olympus FV10i) of Mitosin. U2OS cells were fixed by 4% PFA and costained with Mitosin 14C10 1D8 AB (Red; cat# GTX70137; 1:500 Ab dilution) and alpha-tubulin rabbit polyclonal AB (Green; cat# GTX112141; 1:500 Ab dilution ), a spindle marker. DAPI (blue), chromosomes. Scale bar, 5 um
Confocal immunofluorescence staining (Olympus FV10i) of Mitosin. U2OS cells were fixed by 4% PFA and costained with Mitosin 14C10 1D8 AB (Red; cat# GTX70137; 1:500 Ab dilution) and alpha-tubulin rabbit polyclonal AB (Green; cat# GTX112141; 1:500 Ab dilution ), a spindle marker. DAPI (blue), chromosomes. Scale bar, 5 um

CENPF antibody [14C10 1D8]

GTX70137
GeneTex
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetCENPF
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Overview

  • Supplier
    GeneTex
  • Product Name
    CENPF antibody [14C10 1D8]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    14C10 1D8
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID1063
  • Target name
    CENPF
  • Target description
    centromere protein F
  • Target synonyms
    CENF, CILD31, PRO1779, STROMS, hcp-1, centromere protein F, AH antigen, CENP-F kinetochore protein, cell-cycle-dependent 350K nuclear protein, centromere protein F, 350/400kDa, kinetochore protein CENPF, mitosin
  • Host
    Mouse
  • Isotype
    IgG2a
  • Protein IDP49454
  • Protein Name
    Centromere protein F
  • Scientific Description
    This gene encodes a protein that associates with the centromere-kinetochore complex. The protein is a component of the nuclear matrix during the G2 phase of interphase. In late G2 the protein associates with the kinetochore and maintains this association through early anaphase. It localizes to the spindle midzone and the intracellular bridge in late anaphase and telophase, respectively, and is thought to be subsequently degraded. The localization of this protein suggests that it may play a role in chromosome segregation during mitotis. It is thought to form either a homodimer or heterodimer. Autoantibodies against this protein have been found in patients with cancer or graft versus host disease. [provided by RefSeq, Jul 2008]
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

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