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Western Blot analysis of HepG2 cells using Choactase Polyclonal Antibody
Western Blot analysis of HepG2 cells using Choactase Polyclonal Antibody
Western Blot analysis of HepG2 cells using Choactase Polyclonal Antibody

CHAT Antibody

CSB-PA001637
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetCHAT
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Overview

  • Supplier
    Cusabio
  • Product Name
    CHAT Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1103
  • Target name
    CHAT
  • Target description
    choline O-acetyltransferase
  • Target synonyms
    acetyl CoA:choline O-acetyltransferase; CHOACTASE; choline acetylase; choline O-acetyltransferase; CMS1A; CMS1A2; CMS6
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP28329
  • Protein Name
    Choline O-acetyltransferase
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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SHSY5Y cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with ChAT Polyclonal Antibody(bs-0042R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
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