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ProductsBack/Goat anti-Choline acetyltransferase

Goat anti-Choline acetyltransferase

EB06636
Everest Biotech
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetCHAT
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-Choline acetyltransferase Antibody
  • Delivery Days Customer
    5
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    Pep-ELISA, WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID1103
  • Target name
    CHAT
  • Target description
    choline O-acetyltransferase
  • Target synonyms
    acetyl CoA:choline O-acetyltransferase; CHOACTASE; choline acetylase; choline O-acetyltransferase; CMS1A; CMS1A2; CMS6
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_066264.3; NP_065574.3; NP_001136405.1. Purification: Antigen affinity purified. Names and symbols: CHAT; choline acetyltransferase; CMS1A; CMS1A2; acetyl CoA:choline O-acetyltransferase
  • Reactivity Supplier
    Human, Dog, Pig, Cow
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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SHSY5Y cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with ChAT Polyclonal Antibody(bs-0042R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
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