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IHC-P analysis of 4% PFA-fixed mouse caudate nucleus section using GTX85450 Choline Acetyltransferase antibody. The green staining is low-level GFP immunoreactivity in this transgenic animal. Red : Primary antibody
IHC-P analysis of 4% PFA-fixed mouse caudate nucleus section using GTX85450 Choline Acetyltransferase antibody. The green staining is low-level GFP immunoreactivity in this transgenic animal. Red : Primary antibody
IHC-P analysis of 4% PFA-fixed mouse caudate nucleus section using GTX85450 Choline Acetyltransferase antibody. The green staining is low-level GFP immunoreactivity in this transgenic animal. Red : Primary antibody

Choline Acetyltransferase antibody

GTX85450
GeneTex
ApplicationsImmunoFluorescence, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetCHAT
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Overview

  • Supplier
    GeneTex
  • Product Name
    Choline Acetyltransferase antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    IHC: 1:1000-1:2000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    100 ug/ml
  • Conjugate
    Unconjugated
  • Gene ID1103
  • Target name
    CHAT
  • Target description
    choline O-acetyltransferase
  • Target synonyms
    acetyl CoA:choline O-acetyltransferase; CHOACTASE; choline acetylase; choline O-acetyltransferase; CMS1A; CMS1A2; CMS6
  • Host
    Chicken
  • Isotype
    IgY
  • Protein IDP28329
  • Protein Name
    Choline O-acetyltransferase
  • Scientific Description
    This gene encodes an enzyme which catalyzes the biosynthesis of the neurotransmitter acetylcholine. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimers disease. Polymorphisms in this gene have been associated with Alzheimers disease and mild cognitive impairment. Mutations in this gene are associated with congenital myasthenic syndrome associated with episodic apnea. Multiple transcript variants encoding different isoforms have been found for this gene, and some of these variants have been shown to encode more than one isoform. [provided by RefSeq, May 2010]
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 2. IHC analysis of CHAT using anti-CHAT antibody (A01192-3). CHAT was detected in paraffin-embedded section of mouse brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1microg/ml rabbit anti-CHAT Antibody (A01192-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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