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Collagen I antibody

GTX44085
GeneTex
ApplicationsELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin, RadioImmunoAssay
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Product group Antibodies
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Overview

  • Supplier
    GeneTex
  • Product Name
    Collagen I antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    IHC-P: 1:500. IHC-Fr: 1:40. ELISA: 1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin, RadioImmunoAssay
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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This image was kindly submitted by Kandy Velazquez of USC School of Medicine._x000D_ Mouse adipose tissue lysates probed with Collagen I Polyclonal Antibody, unconjugated (bs-10423R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody at 1:2000 for 60 minutes at room temperature.
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Figure 1. Western blot analysis of Collagen I using anti-Collagen I antibody (PB9938). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: rat skin tissue lysates, Lane 3: rat lung tissue lysates, Lane 4: mouse skin tissue lysates, Lane 5: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Collagen I antigen affinity purified polyclonal antibody (Catalog # PB9938) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Collagen I at approximately 130-180 kDa. The expected band size for Collagen I is at 139 kDa.
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