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GeneTex anti collagen II antibody (GTX20300, 1:400, 45 min RT) showed moderate staining of in FFPE human bronchiolar cartilage (shown in image). Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100oC - 20 minutes for antigen retrieval.
GeneTex anti collagen II antibody (GTX20300, 1:400, 45 min RT) showed moderate staining of in FFPE human bronchiolar cartilage (shown in image). Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100oC - 20 minutes for antigen retrieval.
GeneTex anti collagen II antibody (GTX20300, 1:400, 45 min RT) showed moderate staining of in FFPE human bronchiolar cartilage (shown in image). Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100oC - 20 minutes for antigen retrieval.

Collagen II antibody

GTX20300
GeneTex
ApplicationsDot Blot, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Other Application
Product group Antibodies
ReactivityBovine, Human, Rat
TargetCOL2A1
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Overview

  • Supplier
    GeneTex
  • Product Name
    Collagen II antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:1000-1:10000. IHC-P: 1:50-1:400. IP: 1:100. ELISA: 1:5000-1:50000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Dot Blot, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Other Application
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID1280
  • Target name
    COL2A1
  • Target description
    collagen type II alpha 1 chain
  • Target synonyms
    ACG2, ANFH, ANFH1, AOM, COL11A3, EDMMD, LCPD, OSCDP, PLSDT, SEDC, SEDSTN, SEMDSTWK, SMDALG, STL1, VPED, collagen alpha-1(II) chain, alpha-1 type II collagen, arthroophthalmopathy, progressive (Stickler syndrome), cartilage collagen, chondrocalcin, collagen II, alpha-1 polypeptide, collagen, type II, alpha 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP02458
  • Protein Name
    Collagen alpha-1(II) chain
  • Scientific Description
    Collagens are highly conserved throughout evolution and are characterized by an uninterrupted Glycine-X-Y triplet repeat that is a necessary part of the triple helical structure. For these reasons it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. This preparation results in a native conformation of the protein.
  • Reactivity
    Bovine, Human, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Wu KC, Weng HK, Hsu YS, et al. Aqueous extract of Arctium lappa L. root (burdock) enhances chondrogenesis in human bone marrow-derived mesenchymal stem cells. BMC Complement Med Ther. 2020,20(1):364. doi: 10.1186/s12906-020-03158-1
    Read this paper
  • Chang YH, Wu KC, Wang CC, et al. Enhanced chondrogenesis of human umbilical cord mesenchymal stem cells in a gelatin honeycomb scaffold. J Biomed Mater Res A. 2020,108(10):2069-2079. doi: 10.1002/jbm.a.36966
    Read this paper
  • Wang Z, Li K, Sun H, et al. Icariin promotes stable chondrogenic differentiation of bone marrow mesenchymal stem cells in self‑assembling peptide nanofiber hydrogel scaffolds. Mol Med Rep. 2018,17(6):8237-8243. doi: 10.3892/mmr.2018.8913
    Read this paper
  • Maraldi T, Guida M, Zavatti M, et al. Nuclear Nox4 Role in Stemness Power of Human Amniotic Fluid Stem Cells. Oxid Med Cell Longev. 2015,2015:101304. doi: 10.1155/2015/101304
    Read this paper
  • Wang ZC, Sun HJ, Li KH, et al. Icariin promotes directed chondrogenic differentiation of bone marrow mesenchymal stem cells but not hypertrophy in vitro. Exp Ther Med. 2014,8(5):1528-1534.
    Read this paper

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Figure 1. Western blot analysis of COL2A1 using anti-COL2A1 antibody (A00517-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat cartilage tissue lysates, Lane 2: mouse cartilage tissue lysates, Lane 3: mouse auricular cartilage lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-COL2A1 antigen affinity purified polyclonal antibody (Catalog # A00517-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for COL2A1 at approximately 180-190 kDa. The expected band size for COL2A1 is at 142 kDa.
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