More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/Collagen II antibody
GeneTex anti collagen II antibody (GTX20300, 1:400, 45 min RT) showed moderate staining of in FFPE human bronchiolar cartilage (shown in image). Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100oC - 20 minutes for antigen retrieval.
GeneTex anti collagen II antibody (GTX20300, 1:400, 45 min RT) showed moderate staining of in FFPE human bronchiolar cartilage (shown in image). Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100oC - 20 minutes for antigen retrieval.
GeneTex anti collagen II antibody (GTX20300, 1:400, 45 min RT) showed moderate staining of in FFPE human bronchiolar cartilage (shown in image). Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100oC - 20 minutes for antigen retrieval.

Collagen II antibody

GTX20300
GeneTex
ApplicationsDot Blot, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Other Application
DownloadDatasheet
Product group Antibodies
TargetCOL2A1
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    GeneTex
  • Product Name
    Collagen II antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:1000-1:10000. IHC-P: 1:50-1:400. IP: 1:100. ELISA: 1:5000-1:50000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Dot Blot, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Other Application
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID1280
  • Target name
    COL2A1
  • Target description
    collagen type II alpha 1 chain
  • Target synonyms
    alpha-1 type II collagen; ANFH; AOM; arthroophthalmopathy, progressive (Stickler syndrome); cartilage collagen; chondrocalcin; COL11A3; collagen alpha-1(II) chain; collagen II, alpha-1 polypeptide; collagen, type II, alpha 1; SEDC; STL1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP02458
  • Protein Name
    Collagen alpha-1(II) chain
  • Scientific Description
    Collagens are highly conserved throughout evolution and are characterized by an uninterrupted Glycine-X-Y triplet repeat that is a necessary part of the triple helical structure. For these reasons it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. This preparation results in a native conformation of the protein.
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Aqueous extract of Arctium lappa L. root (burdock) enhances chondrogenesis in human bone marrow-derived mesenchymal stem cells. Wu KC et al., 2020 Nov 23, BMC Complement Med Ther
    Read more
  • Enhanced chondrogenesis of human umbilical cord mesenchymal stem cells in a gelatin honeycomb scaffold. Chang YH et al., 2020 Oct, J Biomed Mater Res A
    Read more
  • Icariin promotes stable chondrogenic differentiation of bone marrow mesenchymal stem cells in self-assembling peptide nanofiber hydrogel scaffolds. Wang Z et al., 2018 Jun, Mol Med Rep
    Read more
  • Nuclear Nox4 Role in Stemness Power of Human Amniotic Fluid Stem Cells. Maraldi T et al., 2015, Oxid Med Cell Longev
    Read more
  • Icariin promotes directed chondrogenic differentiation of bone marrow mesenchymal stem cells but not hypertrophy in vitro. Wang ZC et al., 2014 Nov, Exp Ther Med
    Read more

Related products

Product group Antibodies
RayBiotech
Anti-Mouse COL2A1 Antibody144-01560
ApplicationsImmunoFluorescence, Western Blot
TargetCOL2A1
  • SizePrice
Product group Antibodies
Absolute Antibody
Anti-Collagen type II [M2139], Mouse IgG1, kappaAB02802-1.1
ApplicationsELISA, ImmunoHistoChemistry, Neutralisation/Blocking, Other Application
TargetCOL2A1
  • SizePrice
Product group Antibodies
References
GeneTex
Collagen II antibodyGTX44078
ApplicationsImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin, RadioImmunoAssay
TargetCOL2A1
  • SizePrice
Product group Antibodies
Biosynth
Collagen Type II antibody10R-C135A
  • SizePrice
Figure 1. Western blot analysis of COL2A1 using anti-COL2A1 antibody (A00517-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat cartilage tissue lysates, Lane 2: mouse cartilage tissue lysates, Lane 3: mouse auricular cartilage lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-COL2A1 antigen affinity purified polyclonal antibody (Catalog # A00517-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for COL2A1 at approximately 180-190 kDa. The expected band size for COL2A1 is at 142 kDa.
Product group Antibodies
Boster Bio
Anti-Collagen II/COL2A1 Antibody Picoband(r)A00517-1-CARRIER-FREE
ApplicationsWestern Blot
TargetCOL2A1
  • SizePrice
Product group Antibodies
Assay Genie
IPO4 AntibodyCAB15600
TargetCOL2A1
  • SizePrice
Product group Antibodies
Biomatik
Cross Linked C-Telopeptide Of Type II Collagen (CTXII) Polyclonal AntibodyCAU27370
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetCOL2A1
  • SizePrice
Immunohistochemical analysis of paraffin-embedded Human Lung Carcinoma Tissue using Collagen II Mouse mAb diluted at 1:200.
Product group Antibodies
Cusabio
COL2A1 Monoclonal AntibodyCSB-MA186977
ApplicationsELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetCOL2A1
  • SizePrice
Product group Antibodies
References
GeneTex
Collagen II antibody [N1], N-termGTX100829
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetCOL2A1
  • SizePrice
Product group Antibodies
GeneTex
Collagen II antibody [HL1772]GTX637424
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetCOL2A1
  • SizePrice