
GeneTex anti collagen II antibody (GTX20300, 1:400, 45 min RT) showed moderate staining of in FFPE human bronchiolar cartilage (shown in image). Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100oC - 20 minutes for antigen retrieval.
Collagen II antibody
GTX20300
ApplicationsDot Blot, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Other Application
Product group Antibodies
ReactivityBovine, Human, Rat
TargetCOL2A1
Overview
- SupplierGeneTex
- Product NameCollagen II antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 1:1000-1:10000. IHC-P: 1:50-1:400. IP: 1:100. ELISA: 1:5000-1:50000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsDot Blot, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Other Application
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID1280
- Target nameCOL2A1
- Target descriptioncollagen type II alpha 1 chain
- Target synonymsACG2, ANFH, ANFH1, AOM, COL11A3, EDMMD, LCPD, OSCDP, PLSDT, SEDC, SEDSTN, SEMDSTWK, SMDALG, STL1, VPED, collagen alpha-1(II) chain, alpha-1 type II collagen, arthroophthalmopathy, progressive (Stickler syndrome), cartilage collagen, chondrocalcin, collagen II, alpha-1 polypeptide, collagen, type II, alpha 1
- HostRabbit
- IsotypeIgG
- Protein IDP02458
- Protein NameCollagen alpha-1(II) chain
- Scientific DescriptionCollagens are highly conserved throughout evolution and are characterized by an uninterrupted Glycine-X-Y triplet repeat that is a necessary part of the triple helical structure. For these reasons it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. This preparation results in a native conformation of the protein.
- ReactivityBovine, Human, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
References
- Aqueous extract of Arctium lappa L. root (burdock) enhances chondrogenesis in human bone marrow-derived mesenchymal stem cells. Wu KC et al., 2020 Nov 23, BMC Complement Med TherRead this paper
- Enhanced chondrogenesis of human umbilical cord mesenchymal stem cells in a gelatin honeycomb scaffold. Chang YH et al., 2020 Oct, J Biomed Mater Res ARead this paper
- Icariin promotes stable chondrogenic differentiation of bone marrow mesenchymal stem cells in self-assembling peptide nanofiber hydrogel scaffolds. Wang Z et al., 2018 Jun, Mol Med RepRead this paper
- Nuclear Nox4 Role in Stemness Power of Human Amniotic Fluid Stem Cells. Maraldi T et al., 2015, Oxid Med Cell LongevRead this paper
- Icariin promotes directed chondrogenic differentiation of bone marrow mesenchymal stem cells but not hypertrophy in vitro. Wang ZC et al., 2014 Nov, Exp Ther MedRead this paper






![Collagen II antibody [N1], N-term detects Collagen II protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Collagen II antibody [N1], N-term (GTX100829) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX100829/GTX100829_40751_20170510_IHC-P_R_w_23060100_562.webp)
![HepG2 whole cell extract and conditioned medium (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Collagen II antibody [HL1772] (GTX637424) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637424/GTX637424_44963_20230224_WB_Fraction_23030219_353.webp)