GeneTex anti collagen II antibody (GTX20300, 1:400, 45 min RT) showed moderate staining of in FFPE human bronchiolar cartilage (shown in image). Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100oC - 20 minutes for antigen retrieval.
GeneTex anti collagen II antibody (GTX20300, 1:400, 45 min RT) showed moderate staining of in FFPE human bronchiolar cartilage (shown in image). Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100oC - 20 minutes for antigen retrieval.
Collagen II antibody
GTX20300
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Human, Mouse, Rat, Sheep
TargetCOL2A1
Overview
- SupplierGeneTex
- Product NameCollagen II antibody
- Delivery Days Customer9
- Antibody SpecificitySome class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type II collagens an
- Application Supplier NoteWB: 1:1000-1:10000. IHC-P: 1:50-1:400. IP: 1:100. ELISA: 1:5000-1:50000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 mg/ml
- ConjugateUnconjugated
- FormulationLiquid
- Gene ID1280
- Target nameCOL2A1
- Target descriptioncollagen type II alpha 1 chain
- Target synonymsalpha-1 type II collagen; ANFH; AOM; arthroophthalmopathy, progressive (Stickler syndrome); cartilage collagen; chondrocalcin; COL11A3; collagen alpha-1(II) chain; collagen II, alpha-1 polypeptide; collagen, type II, alpha 1; SEDC; STL1
- HostRabbit
- IsotypeIgG
- Protein IDP02458
- Protein NameCollagen alpha-1(II) chain
- Scientific DescriptionCollagens are highly conserved throughout evolution and are characterized by an uninterrupted Glycine-X-Y triplet repeat that is a necessary part of the triple helical structure. For these reasons it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. This preparation results in a native conformation of the protein.
- ReactivityBovine, Human, Mouse, Rat, Sheep
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Aqueous extract of Arctium lappa L. root (burdock) enhances chondrogenesis in human bone marrow-derived mesenchymal stem cells. Wu KC et al., 2020 Nov 23, BMC Complement Med TherRead more
- Enhanced chondrogenesis of human umbilical cord mesenchymal stem cells in a gelatin honeycomb scaffold. Chang YH et al., 2020 Oct, J Biomed Mater Res ARead more
- Icariin promotes stable chondrogenic differentiation of bone marrow mesenchymal stem cells in self-assembling peptide nanofiber hydrogel scaffolds. Wang Z et al., 2018 Jun, Mol Med RepRead more
- Nuclear Nox4 Role in Stemness Power of Human Amniotic Fluid Stem Cells. Maraldi T et al., 2015, Oxid Med Cell LongevRead more
- Icariin promotes directed chondrogenic differentiation of bone marrow mesenchymal stem cells but not hypertrophy in vitro. Wang ZC et al., 2014 Nov, Exp Ther MedRead more