GeneTex anti collagen III antibody (GTX26580, 1:400, 45 min RT) showed strong staining in FFPE sections of human skin(left, dermis) with moderate to strong red staining and testis (right) where strong staining was observed within connective tissue between seminiferous tubules. The antibody showed strong extracellular staining within connective tissues across many organs with minimal background staining. Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100oC - 20 minutes for antigen retrieval.
GeneTex anti collagen III antibody (GTX26580, 1:400, 45 min RT) showed strong staining in FFPE sections of human skin(left, dermis) with moderate to strong red staining and testis (right) where strong staining was observed within connective tissue between seminiferous tubules. The antibody showed strong extracellular staining within connective tissues across many organs with minimal background staining. Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100oC - 20 minutes for antigen retrieval.
Collagen III antibody (Biotin)
GTX26580
ApplicationsImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Human
TargetCOL3A1
Overview
- SupplierGeneTex
- Product NameCollagen III antibody (Biotin)
- Delivery Days Customer9
- Antibody SpecificitySome class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type III collagens a
- Application Supplier NoteWB: 1:1000-1:5000. IHC-P: 1:200-1:1000. IP: 1:100. ELISA: 1:10000-1:50000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 mg/ml
- ConjugateBiotin
- Gene ID1281
- Target nameCOL3A1
- Target descriptioncollagen type III alpha 1 chain
- Target synonymsalpha1 (III) collagen; alpha-1 type III collagen; collagen alpha-1(III) chain; collagen, fetal; collagen, type III, alpha 1; EDS4A; EDSVASC; Ehlers-Danlos syndrome type IV, autosomal dominant; PMGEDSV
- HostRabbit
- IsotypeIgG
- Protein IDP02461
- Protein NameCollagen alpha-1(III) chain
- Scientific DescriptionThis antibody is well suited to detect extracellular matrix proteins in normal as well as disease state tissues. Disruption of tissue organization is the hallmark of neoplasia. Malignant lesions can be distinguished from benign by examining the breakdown of basement membranes and loss of 3-dimensional architecture. Malignant cells are presumed to use matrix metalloproteases to degrade barriers created by the extracellular matrix, which then allows metastasis to occur. Collagenases, stomelysins and gelatinases can collectively degrade all of the various components of the extracellular matrix, including fibrillar and non-fibrillar collagens and basement membrane glycoproteins.
- ReactivityBovine, Human
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203