GeneTex anti collagen III antibody (GTX27778, 45 min RT) showed strong staining in FFPE sections of human skin(left, dermis) with moderate to strong red staining and testis (right) where strong staining was observed within connective tissue between seminiferous tubules. The antibody showed strong extracellular staining within connective tissues across many organs with minimal background staining. Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100oC - 20 minutes for antigen retrieval.
GeneTex anti collagen III antibody (GTX27778, 45 min RT) showed strong staining in FFPE sections of human skin(left, dermis) with moderate to strong red staining and testis (right) where strong staining was observed within connective tissue between seminiferous tubules. The antibody showed strong extracellular staining within connective tissues across many organs with minimal background staining. Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100oC - 20 minutes for antigen retrieval.
Collagen III antibody
GTX27778
ApplicationsDot Blot, Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin, Other Application
Product group Antibodies
ReactivityBovine, Human, Mouse, Porcine
TargetCOL3A1
Overview
- SupplierGeneTex
- Product NameCollagen III antibody
- Delivery Days Customer9
- Antibody SpecificitySome class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type III collagens a
- Application Supplier NoteWB: 1:1000-1:10000. IHC-P: 1:50-1:200. IP: 1:100. ELISA: 1:5000-1:50000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsDot Blot, Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin, Other Application
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1.16 mg/ml
- ConjugateUnconjugated
- Gene ID1281
- Target nameCOL3A1
- Target descriptioncollagen type III alpha 1 chain
- Target synonymsalpha1 (III) collagen; alpha-1 type III collagen; collagen alpha-1(III) chain; collagen, fetal; collagen, type III, alpha 1; EDS4A; EDSVASC; Ehlers-Danlos syndrome type IV, autosomal dominant; PMGEDSV
- HostRabbit
- IsotypeIgG
- Protein IDP02461
- Protein NameCollagen alpha-1(III) chain
- Scientific DescriptionThese antibodies are well suited to detect extracellular matrix proteins in normal as well as disease state tissues. Disruption of tissue organization is the hallmark of neoplasia. Malignant lesions can be distinguished from benign by examining the breakdown of basement membranes and loss of 3-dimensional architecture. Malignant cells are presumed to use matrix metalloproteases to degrade barriers created by the extracellular matrix, which then allows metastasis to occur. Collagenases, stomelysins and gelatinases can collectively degrade all of the various components of the extracellular matrix, including fibrillar and non-fibrillar collagens and basement membrane glycoproteins.
- ReactivityBovine, Human, Mouse, Porcine
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
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- Candesartan ameliorates arsenic-induced hypertensive vascular remodeling by regularizing angiotensin II and TGF-beta signaling in rats. Khuman MW et al., 2016 Dec 30, ToxicologyRead more
- Saikosaponin d induces cell death through caspase-3-dependent, caspase-3-independent and mitochondrial pathways in mammalian hepatic stellate cells. Chen MF et al., 2016 Jul 26, BMC CancerRead more
- Vasoprotective effects of urocortin 1 against atherosclerosis in vitro and in vivo. Hasegawa A et al., 2014, PLoS OneRead more
- The behavior of ligament cells cultured on elastin and collagen scaffolds. Mizutani N et al., 2014 Mar, J Artif OrgansRead more
- Impact of Smad3 loss of function on scarring and adhesion formation during tendon healing. Katzel EB et al., 2011 May, J Orthop ResRead more