GeneTex anti collagen III antibody (GTX27778, 45 min RT) showed strong staining in FFPE sections of human skin(left, dermis) with moderate to strong red staining and testis (right) where strong staining was observed within connective tissue between seminiferous tubules. The antibody showed strong extracellular staining within connective tissues across many organs with minimal background staining. Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100oC - 20 minutes for antigen retrieval.
GeneTex anti collagen III antibody (GTX27778, 45 min RT) showed strong staining in FFPE sections of human skin(left, dermis) with moderate to strong red staining and testis (right) where strong staining was observed within connective tissue between seminiferous tubules. The antibody showed strong extracellular staining within connective tissues across many organs with minimal background staining. Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100oC - 20 minutes for antigen retrieval.
Collagen III antibody
GTX27778
ApplicationsDot Blot, Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin, Other Application
Product group Antibodies
TargetCOL3A1
Overview
- SupplierGeneTex
- Product NameCollagen III antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 1:1000-1:10000. IHC-P: 1:50-1:200. IP: 1:100. ELISA: 1:5000-1:50000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsDot Blot, Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin, Other Application
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1.16 mg/ml
- ConjugateUnconjugated
- Gene ID1281
- Target nameCOL3A1
- Target descriptioncollagen type III alpha 1 chain
- Target synonymsEDS4A, EDSVASC, PMGEDSV, collagen alpha-1(III) chain, Ehlers-Danlos syndrome type IV, autosomal dominant, alpha-1 type III collagen, alpha1 (III) collagen, collagen, fetal, collagen, type III, alpha 1
- HostRabbit
- IsotypeIgG
- Protein IDP02461
- Protein NameCollagen alpha-1(III) chain
- Scientific DescriptionThese antibodies are well suited to detect extracellular matrix proteins in normal as well as disease state tissues. Disruption of tissue organization is the hallmark of neoplasia. Malignant lesions can be distinguished from benign by examining the breakdown of basement membranes and loss of 3-dimensional architecture. Malignant cells are presumed to use matrix metalloproteases to degrade barriers created by the extracellular matrix, which then allows metastasis to occur. Collagenases, stomelysins and gelatinases can collectively degrade all of the various components of the extracellular matrix, including fibrillar and non-fibrillar collagens and basement membrane glycoproteins.
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Tong R, Yang B, Xiao H, et al. KCTD11 inhibits growth and metastasis of hepatocellular carcinoma through activating Hippo signaling. Oncotarget. 2017,8(23):37717-37729. doi: 10.18632/oncotarget.17145Read this paper
- Khuman MW, Harikumar SK, Sadam A, et al. Candesartan ameliorates arsenic-induced hypertensive vascular remodeling by regularizing angiotensin II and TGF-beta signaling in rats. Toxicology. 2016,374:29-41. doi: 10.1016/j.tox.2016.11.015Read this paper
- Chen MF, Huang SJ, Huang CC, et al. Saikosaponin d induces cell death through caspase-3-dependent, caspase-3-independent and mitochondrial pathways in mammalian hepatic stellate cells. BMC Cancer. 2016,16:532. doi: 10.1186/s12885-016-2599-0Read this paper
- Hasegawa A, Sato K, Shirai R, et al. Vasoprotective effects of urocortin 1 against atherosclerosis in vitro and in vivo. PLoS One. 2014,9(12):e110866. doi: 10.1371/journal.pone.0110866Read this paper
- Mizutani N, Kageyama S, Yamada M, et al. The behavior of ligament cells cultured on elastin and collagen scaffolds. J Artif Organs. 2014,17(1):50-9. doi: 10.1007/s10047-013-0736-yRead this paper
- Katzel EB, Wolenski M, Loiselle AE, et al. Impact of Smad3 loss of function on scarring and adhesion formation during tendon healing. J Orthop Res. 2011,29(5):684-93. doi: 10.1002/jor.21235Read this paper
Datasheet
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