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ProductsBack/Collagen III antibody [FH-7A]
IHC-Fr analysis of rat skin tissue using GTX26310 Collagen III antibody [FH-7A] at 1:8,000.
IHC-Fr analysis of rat skin tissue using GTX26310 Collagen III antibody [FH-7A] at 1:8,000.
IHC-Fr analysis of rat skin tissue using GTX26310 Collagen III antibody [FH-7A] at 1:8,000.

Collagen III antibody [FH-7A]

GTX26310
GeneTex
ApplicationsDot Blot, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Rabbit, Rat
TargetCOL3A1
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Overview

  • Supplier
    GeneTex
  • Product Name
    Collagen III antibody [FH-7A]
  • Delivery Days Customer
    9
  • Application Supplier Note
    IHC-Fr: 1:4,000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Dot Blot, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    FH-7A
  • Conjugate
    Unconjugated
  • Gene ID1281
  • Target name
    COL3A1
  • Target description
    collagen type III alpha 1 chain
  • Target synonyms
    EDS4A, EDSVASC, PMGEDSV, collagen alpha-1(III) chain, Ehlers-Danlos syndrome type IV, autosomal dominant, alpha-1 type III collagen, alpha1 (III) collagen, collagen, fetal, collagen, type III, alpha 1
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP02461
  • Protein Name
    Collagen alpha-1(III) chain
  • Scientific Description
    Collagen III is a fibrillar collagen that is found in extensible connective tissues such as skin, lung, and the vascular system, frequently in association with type I Collagen. Mutations in the gene are associated with Ehlers Danlos syndrome type IV, and with aortic and arterial aneurysms. Although alternate transcripts have been detected for this gene, they are the result of mutations; these mutations alter splicing, often leading to the exclusion of multiple exons.
  • Reactivity
    Human, Rabbit, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

References

  • Fabrication of bFGF/polydopamine-loaded PEEK implants for improving soft tissue integration by upregulating Wnt/beta-catenin signaling.
    Read this paper
  • Recombinant DTbeta4-inspired porous 3D vascular graft enhanced antithrombogenicity and recruited circulating CD93(+)/CD34(+) cells for endothelialization.
    Read this paper
  • Tissue-Material Integration and Biostimulation Study of Collagen Acellular Matrices. Quintero Sierra LA et al., 2022 Jun, Tissue Eng Regen Med
    Read this paper
  • The effect of prior long-term recellularization with keratocytes of decellularized porcine corneas implanted in a rabbit anterior lamellar keratoplasty model. Fernandez-Perez J et al., 2021, PLoS One
    Read this paper
  • Amianthoid transformation of costal cartilage matrix in children with pectus excavatum and pectus carinatum. Kurkov A et al., 2021, PLoS One
    Read this paper
  • Artificial dermal substitutes for tissue regeneration: comparison of the clinical outcomes and histological findings of two templates. De Francesco F et al., 2020 Aug, J Int Med Res
    Read this paper
  • Inhibition of CD44 induces apoptosis, inflammation, and matrix metalloproteinase expression in tendinopathy. Wu PT et al., 2019 Dec 27, J Biol Chem
    Read this paper
  • Developing Raman spectroscopy as a diagnostic tool for label-free antigen detection. Lewis AT et al., 2018 Feb, J Biophotonics
    Read this paper
  • Structure-function relations in an elastase-induced mouse model of emphysema. Hamakawa H et al., 2011 Sep, Am J Respir Cell Mol Biol
    Read this paper

Datasheet

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Figure 1. Western blot analysis of Collagen III/COL3A1 using anti-Collagen III/COL3A1 antibody (A00788-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse skin tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Collagen III/COL3A1 antigen affinity purified polyclonal antibody (Catalog # A00788-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Collagen III/COL3A1 at approximately 200 kDa. The expected band size for Collagen III/COL3A1 is at 139 kDa.
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GeneTex anti collagen III antibody (GTX27778, 45 min RT) showed strong staining in FFPE sections of human skin(left, dermis) with moderate to strong red staining and testis (right) where strong staining was observed within connective tissue between seminiferous tubules. The antibody showed strong extracellular staining within connective tissues across many organs with minimal background staining. Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100oC - 20 minutes for antigen retrieval.
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